Bioethanol is one of the most promising alternatives to fossil fuels and can be produced from various renewable sources rich in carbohydrates. A priority direction of biotechnological research is the development of new resource-saving technologies of bioethanol production. For effective bioconversion of carbohydrate-containing raw materials into bioethanol, it is advisable to optimise the technological parameters of fermenting highly concentrated wort obtained from grain raw materials. Laboratory experimental studies of the samples according to the newly developed technology have helped to substuntiate the technological parameters of fermenting highly concentrated wort and establishing the regular patterns in how the ethanol concentration in fermented washes changes depending on the initial concentration of dry matter in the wort, the industrial yeast concentration, and the fermentation temperature. Grain wort was fermented with the osmophilic thermotolerant race of distiller’s yeast Saccharomyces cerevisiae DО–16 (IMB Y-5099) at 32–37°C. The initial concentration of dry matter was 26–30%. To optimise the technological parameters of the process of fermenting highly concentrated grain wort, an experiment was designed, which resulted in building mathematical models. To obtain the second-order regression equations in natural form, the basic level of each technological parameter of the process and its variability interval were determined. The mathematical model developed makes it possible to calculate the ethanol concentration depending on the initial concentration of the wort, the concentration of industrial yeast, and the fermentation temperature. Fermentation of highly concentrated wort for bioethanol production was optimised according to the equations of the mathematical model. It has been established that to synthesise the maximum alcohol concentration in washes using the highly concentrated wort fermentation technology, the wort concentration should be 30% of dry matter, the concentration of industrial yeast 40 million/cm³, and the temperature of the main fermentation 35°C.