Relevance. The most well-known biologically active substances are natural antioxidants, which consist of: polyphenols, flavonoids, various aromatic hydroxy acids, anthocyanins, vitamins C and E, carotenoids and other compounds. Raspberries are rich in phenolic phytochemicals, especially flavonoids, such as anthocyanin pigments, which give raspberries a dark red color. The aim of the study is to analyze biologically active substances of raspberries of Kazakhstan breeding in laboratory conditions. Research objectives: theoretical analysis, collection and structuring of laboratory data on biologically active substances, appropriate conclusion.Methods. The physicochemical properties and chemical indicators in the various raspberry varieties of the Kazakhstan selection were studied, such as: Anar, Arai and Salem. Mostly the recognized and available research methods were used. The method of determining the amount of soluble solids is based on using a refractometer. The found value is expressed in units of the mass fraction of sucrose in an aqueous solution of sucrose, which under given conditions has the same refractive index as the analyzed solution, in % (Brix) (GOST 51433: 1999).Results. Physico-chemical parameters were studied: the dry matter content, total sugars and titrated acidity. It is established that among the raspberry varieties considered, Anar variety has the highest values. Anar also has the highest chemical indicators: the sugar acid index, the content of ascorbic acid (vitamin C), the total content of phenols and the total content of flavonoids. The total content of phenols and flavonoids in the varieties Anar (860 µg/ml and 188 µg/ml, respectively), Arai (870 µg/ml and 189 µg/ml, respectively) and Salem (855 µg/ml and 187 µg/ml, respectively) have similar values. Extracts of these raspberries sorts could be advised for the enrichment of the nutritional value composition of food products.