Микроводоросли эпизоона культивируемого моллюска Мytilus galloprovincialis Lam. 1819, фитопланктон и гидролого-гидрохимические характеристики акватории мидийно-устричной фермы (Севастополь, Чёрное море)

Abstract

In mollusk cultivation areas large amount of biomass and metabolites is accumulated. For this reason, biological monitoring in the farming areas, which includes study of microalgae as environmental quality indicators, is of considerable importance. Samples of mussels harvested from collectors at 6 m depth over the period February 2015 – March 2016 have been utilized for studying epizoon microalgae residing on mollusk shells. At the same time, sea water at depths of 0 and 6 m was sampled for determining phytoplankton and hydrochemical parameters of environment in the mussel-and-oyster farm area. Dissolved oxygen, biological oxygen demand after five days of incubation in the dark (BOD5), alkaline permanganate oxidizability, silicates, organic and inorganic forms of nitrogen and phosphorus have been quantified in the water samples using conventional methods. In the epizoon of the mussel shells, 108 taxa of microalgae of four phyla have been identified: 3 species of Сyanoprokaryota, 6 of Dinophyta, 6 of Haptophyta and 93 of Bacillariophyta. The maximum values of the species richness (26) and abundance of microalgae were observed in February (74,78·103 cells·cm-2, t = 9,7 °C) and April 2015 (62,0·103 cells·cm-2, t = 10,3 °C), as well as in January 2016 (65,1·103 cells·cm-2, t = 9,5 °C). The highest biomass was registered in August (0,272 mg·cm-2, t = 25,5 °C). The main contribution to the total abundance was made by the diatoms Tabularia fasciculata while Navicula ramosissima, and cyanobacteria were prevalent in the total biomass. In phytoplankton at the depths of 0 and 6 m, 135 taxa belonging to eight phyla have been found: 2 species of Cyanoprokaryota, 47 of Acillariophyta, 57 of Inophyta, 17 of Haptophyta, 5 of Chlorophyta, 2 of Euglenophyta, 3 of Cryptophyta and 2 of Chrysophyta. The genus Chaetoceros dominated by the number of diatoms species (18). In terms of abundance and biomass, the dinoflagellate Prorocentrum micans and haptophyte Emiliania huxleyi were dominant. The maximum abundance (370·107 cells·m-3) and biomass (7560 mg·m-3) of the phytoplankton were observed in spring and autumn. In total, 213 of microalgae taxa have been identified in the phytoplankton and mussel shell epizoon, with 30 ones being common for both. Furthermore, 26 potentially toxic species and 24 indicator species have been determined, among which 26 ones are betamesosaprobionts, the indicators of moderate level of water pollution. Thermohaline characteristics of water in the mollusk farm area did not exceed those of the long-term observations. At all horizons, the oxygen content was at the level of 93–125 % of saturation. The sea water oxidizability did not exceed the maximum permissible level established by fishery standards. The concentration of nutrients was high with a large fluctuation range, which indicates anthropogenic impact on the water area. The values of the total inorganic nitrogen-to-phosphorus and silicon-to-phosphorus ratios suggested nitrogen and silicon limitations for the microalgae community development from July to December. The mussel epizoon microalgae abundance strongly correlated with water temperature and dissolved oxygen, and a strong correlation of the biomass with inorganic phosphorus was observed, too. Moderate correlations were also found with inorganic phosphorus and organic nitrogen. For the phytoplankton, moderate correlations of abundance with hydrological and hydrochemical characteristics were identified: with nitrates in the surface layer and with temperature, dissolved oxygen, and organic nitrogen in the subsurface water layer. The phytoplankton biomass moderately correlated with the silicate concentration. The hydrological and hydrochemical structure of sea water, especially in the mollusk farming areas, affected species composition and quantitative characteristics of planktonic and benthic microalgae communities.

Species Diversity of Epilithon Diatoms and the Quality of the Waters of the Donuzlav Gulf Ecosystem (Crimea, the Black Sea)

“…In Karantinnaya Bay, as a comparison, the corresponding range was 93-125% [21]. The seasonal variability of concentrations of organic nitrogen and phosphorus was not observed.…”

“…Species diversity of microphytobenthos of the stone substrates in the Donuzlav Gulf are 60 taxa of Bacillariophyta (Table 1), belonging to 3 classes, 18 families and 34 genera. In the diatom flora, marine and cosmopolites dominated, which is typical for the phytobenthos of the Crimean coastal waters of the Black Sea [18,21]. The study of diatoms was carried out on a light microscope of C. Zeiss "Axioskop 40" with software AxioVision Rel.…”

“…The largest biomass was registered in August of 2016 (0.272 mg cm −2 , T = 25.5 • C). Near the area for the cultivation of mussels, 24 species-indicators have been detected, among which 16 are beta-mesosaprobionts [21]. It should be noted that in the microphytobenthos of the Azov, Black and Japanese seas this group of saprobity also dominated [11,13,16,18,19].…”

“…Diatom indices used in biomonitoring of water quality of the mostly of the rivers [4][5][6][7][8][9][10] but very poorly marked on the marine benthic ecosystems [11][12][13][14][15][16][17][18]. Despite the fact that diversity species of the microphytobenthos of the Black Sea is well studied in different ecotopes [18][19][20][21][22]. However, diatoms of microphytobenthos in different seasons of the year have never been studied in the Donuzlav Gulf.…”

“…Despite the fact that diversity species of the microphytobenthos of the Black Sea is well studied in different ecotopes [18][19][20][21][22]. However, diatoms of microphytobenthos in different seasons of the year have never been studied in the Donuzlav Gulf.…”