Search citation statements
Paper Sections
Citation Types
Year Published
Publication Types
Relationship
Authors
Journals
Introduction. Multistrain probiotics often include biocompatible strains, which leads to suppression of microbial viability and, as a result, decreases their efficacy. Therefore, new probiotics should be based on well-matched strains with no antagonism. Study objects and methods. The research featured strains of lactic and propionic acid bacteria from the VNIIPBT collection. The method of direct co-cultivation on dense medium (drop technique) was used to assess the biocompatibility of lactic acid bacteria. Antagonism was detected visually based on signs of suppression after 24 and 48 h after the onset of incubation. Antagonism of the consortia was assessed by the Romanovich method. Results and discussion. The screening resulted in seven promising strains with the specific growth rate of 0.32–0.84 h–1 and the maximum population density ≤ 2.2 billion CFU/cm3. A set of experiments on the strain adaptation mechanisms revealed combinations of strains with the lowest antagonism and competition for the substrate. The research resulted in a four-component consortium of Lactobacillus plantarum 314/8, Lactobacillus helveticus R0052/6, Enterococcus faecium B-2240D, and Propionibacterium freudenreichii subsp. shermanii 103/27. The optimal ratio was the one where the cultures were present in equal proportions. The study also described the biosynthetic properties of the consortium and the ratio of the strains in its composition. The consortium demonstrated a balanced growth, good strain compatibility, and absence of antagonism. The cultivation mode was tested anaerobically on milk whey at 37°C for 24 h (strain ratio = 1:1:1:1). Conclusion. The new consortium proved suitable for industrial production of feed probiotics.
Introduction. Multistrain probiotics often include biocompatible strains, which leads to suppression of microbial viability and, as a result, decreases their efficacy. Therefore, new probiotics should be based on well-matched strains with no antagonism. Study objects and methods. The research featured strains of lactic and propionic acid bacteria from the VNIIPBT collection. The method of direct co-cultivation on dense medium (drop technique) was used to assess the biocompatibility of lactic acid bacteria. Antagonism was detected visually based on signs of suppression after 24 and 48 h after the onset of incubation. Antagonism of the consortia was assessed by the Romanovich method. Results and discussion. The screening resulted in seven promising strains with the specific growth rate of 0.32–0.84 h–1 and the maximum population density ≤ 2.2 billion CFU/cm3. A set of experiments on the strain adaptation mechanisms revealed combinations of strains with the lowest antagonism and competition for the substrate. The research resulted in a four-component consortium of Lactobacillus plantarum 314/8, Lactobacillus helveticus R0052/6, Enterococcus faecium B-2240D, and Propionibacterium freudenreichii subsp. shermanii 103/27. The optimal ratio was the one where the cultures were present in equal proportions. The study also described the biosynthetic properties of the consortium and the ratio of the strains in its composition. The consortium demonstrated a balanced growth, good strain compatibility, and absence of antagonism. The cultivation mode was tested anaerobically on milk whey at 37°C for 24 h (strain ratio = 1:1:1:1). Conclusion. The new consortium proved suitable for industrial production of feed probiotics.
An important aspect of the technology of processing fish raw materials is the development of food products of various directions using modern methods of biotechnological processing. In this regard, in food biotechnology, special attention is paid to biotransformation by promising strains of bacterial starter cultures to produce food products with improved quality characteristics, and in particular with a functional orientation. The use of bacterial starter cultures in the production of fish food products can be a way to extend the shelf life due to the formation of metabolites of their vital activity (acids and bacteriocins), which are the main factor of bioconservation. At the same time, the biochemical activity of bacterial starter cultures will contribute to improving the organoleptic and structural-mechanical properties of raw materials, as well as increasing the nutritional value and synthesis of vitamins and other nutrients, and giving the final product probiotic properties. This trend is a response to the interest of the population of the Russian Federation in probiotic products, which allows to normalize the indigenous microflora of the gastrointestinal tract, thereby strengthening the human body and its immune system as a whole. In this regard, the analysis of data on the justification of the use of bacterial starter cultures for the biotransformation of fish raw materials was carried out, and the necessary strains of microorganisms were selected. The analysis of scientific and technical databases has shown the prospects of scientific developments on the use of bacterial starter cultures as protective microorganisms and the study of the influence of the conditions of cultivation and preservation of bioactivity of a living cell under the influence of physical and chemical factors during the technological process of production and subsequent storage of products, which is of particular importance for the creation of probiotic food products. The conducted research will be used in the construction of mathematical models of the process of biotransformation of fish raw materials as a basis for the development of technological processing parameters for its subsequent use in the formulations of "fast food" products containing functional components, which is justified by the accelerated rhythm of life and the desire of modern society to monitor their nutrition by eating products that support a healthy lifestyle. In food biotechnology, special attention is given to biotransformation of promising strains of bacterial starter cultures for the production of food products with improved quality characteristics and functional orientation. In this regard, the analysis of data on the justification of the use of commercial, potentially commercial fish types for biotransformation by bacterial starter cultures and the selection of the necessary strains of microorganisms, which will be used in the construction of mathematical models of the process of biotransformation of fish raw materials as a basis for the development of technological parameters of processing.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.