Особливості Способу Життя Як Фактор Ризику Розвитку І Прогресування Діабетичної Ретинопатії У хворих На Цукровий Діабет 2-Го Типу

Bykhovets, M.Yu.; Rykov, S. О.; Natrus, L.V.

doi:10.22141/2309-8147.7.1.2019.163000

Cited by 2 publications

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“…Â íàø³é ïîïåðåäí³é ðîáîò³ [17] ìè ïðîâîäèëè àíàë³ç ïîêàçíèê³â ñïîñîáó aeèòòÿ (ÑAE): äîòðèìàííÿ ä³ºòè, ô³çè÷íîãî íàâàíòàaeåííÿ, âaeèâàííÿ âîäè, ôðóêò³â òà îâî÷³â ó ïàö³ºíò³â ³ç ÖÄ2 ³ îñ³á, ÿê³ ç íèìè ñï³âñòàâí³ çà îñíîâíèìè ôåíîòèï³÷íèìè îçíàêàìè, ïðîâåäåíèé íà îñíîâ³ àíêåòóâàííÿ â³äïîâ³äíî âñåñâ³òí³õ ðåêîìåíäàö³¿ çäîðîâîãî õàð÷óâàííÿ òà íóòðèöåâòè÷íèõ äîñë³äaeåíü. Ìè ä³éøëè âèñíîâêó, ùî çâè÷êà äî äîäàòêîâîãî ô³çè÷íîãî íàâàíòàaeåííÿ ³ âaeèâàííÿ 1,5-2 ë âîäè äîñòîâ³ðíî âèùå ó îñ³á áåç ÖÄ.…”

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Behavioral and Genetically Determined Differences in Patients With Type 2 Diabetes Mellitus Complicated by Retinopathy With Different Pparg-Dependent Phenotype

2019

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Relevance. The problem of treatment and prevention of microvascular complications against the background of hyperglycemia is much broader than adherence to an appropriate diet. It is necessary to take into account both the behavioral characteristics of a person and the genetically determined mechanisms of metabolic regulation. Objective to study the behavioral and genetically determined differences in patients with type 2 diabetes mellitus complicated by retinopathy with different PPARG-dependent phenotype. Materials and methods. The study included 101 patients with type 2 diabetes mellitus (T2DM), who, according to the results of an ophthalmological examination, revealed various stages of diabetic retinopathy (DR) according to the ETDRS scale. The control group (CG) included 40 people without diabetes, comparable to patients by gender, age, and body mass index. Gene polymorphism was determined using real-time PCR on an automatic amplifier Gene Amp® PCR System 7500, the fatty acid spectrum (FA) was determined using gas-liquid chromatography, and behavioral and nutritional habits were analyze according to the results of a questionnaire. Results. In patients with type 2 diabetes, the most effective way to correct hyperglycemia at all stages of development of complications was the use of insulin in tablet forms. Carriers of the PPARG gene polymorphism had greater stability in achieving the target blood glucose level. The carriers of the wild genotype Pro12Pro did not differ from the CG in the rationality of nutrition and lifestyle. Carriers of the 12Ala allele were characterized by a neglect of food recommendations at the beginning of the disease, but with the progression of the degree of microvascular complications, the awareness of the need for proper nutrition doubled (P <0.05). Their lifestyle was initially less rational than that of carriers of the wild genotype Pro12Pro, but the course of T2DM and the development of complications in the form of DR by 2–3 times (P <0.05) reduced the desire for a healthy lifestyle.

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Behavioral and Genetically Determined Differences in Patients With Type 2 Diabetes Mellitus Complicated by Retinopathy With Different Pparg-Dependent Phenotype

2019

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The Pathogenetic Rationale the Ways of Experimental Type 2 Diabetes Mellitus Modeling

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Relevance. When modeling experimental type 2 diabetes mellitus (T2DM), various schools and research groups receive significant variability in tissue changes, which is difficult to compare and extrapolate as a specific pathogenic or pharmacological effect. The lack of standard operating procedures agreed upon in the scientific community greatly complicates the interpretation of the result. Therefore, the creation of a uniquely pathogenetic animal model of CD2 in animals is an urgent task. Objective. Determination of the complex of exogenous effects for the most efficient reproduction of the experimental model of T2DM in rats and the pathogenetic justification of the changes in the body to study the central mechanisms of homeostasis regulation and their pharmacological correction. Materials and methods. T2DM was model in rats by feeding a high-fat diet (HFD) with additional streptozotocin induction. The model was evaluated by anthropometric measurements, studies of biochemical parameters, an insulin tolerance test, analysis of the spectrum of fatty acids in the composition of tissues. Statistical data processing was performed with using the IBM SPSS Statistics 23. Results. The obtained data were compared with our studies of the lipid metabolism of patients with varying degrees of diabetic retinopathy on the background of T2DM and analysis of the information content of biochemical markers to assess lipid metabolic disturbances on the background of hyperglycemia in patients. It was found that the simulation in rats of experimental T2DM by a food load of HFD (45%) + fructose 20% for 3 months followed by a single induction of streptozotocin (25 mg/kg) causes changes in lipid and carbohydrate metabolism in animals similar changes which are in the human with a diagnosed long-term diabetes and the development of microvascular complications. Conclusion. We determined the optimal combination of effects and developed a consistent experimental load scheme that allows us to obtain a symptom model of type 2 diabetes in an animal experiment. Using the diagnostic algorithm, which includes physiological and laboratory methods, the degree of damage to organs and systems was determined, a comparison was made with the level of metabolic disorders in patients with T2DM and people without diabetes. The given model is a pathogenetically grounded approach for further study of the central mechanisms of homeostasis regulation and their pharmacological correction

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Особливості Способу Життя Як Фактор Ризику Розвитку І Прогресування Діабетичної Ретинопатії У хворих На Цукровий Діабет 2-Го Типу

Cited by 2 publications

References 1 publication

Behavioral and Genetically Determined Differences in Patients With Type 2 Diabetes Mellitus Complicated by Retinopathy With Different Pparg-Dependent Phenotype

Behavioral and Genetically Determined Differences in Patients With Type 2 Diabetes Mellitus Complicated by Retinopathy With Different Pparg-Dependent Phenotype

The Pathogenetic Rationale the Ways of Experimental Type 2 Diabetes Mellitus Modeling

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