Рantoea agglomerans lipopolysaccharides: structure, functional and biological activity

Abstract

this review analyzed literature data, as well as our own research on lipopolysaccharides (lPs) of gram-negative bacteria, focusing mainly on Pantoea agglomerans, a member of the enterobacteriaceae family. the unique structures of O-specific polysaccharide chains of lPs from Pantoea agglomerans represented by both linear and branched tetra-and pentasaccharide repeating units were described for the first time. the heterogeneity of the lPs molecule itself and the presence of several lPs in the bacterial cell, which differ in the structure of lipids a, O-specific polysaccharide chains, serological activity, as well as endotoxic properties, such as toxicity and pyrogenicity, were shown. such heterogeneity represents one of the mechanisms of lPs multifunctionality. Based on the antigenicity of lPs, serotyping of P. agglomerans strains and their assignment to 10 serogroups were carried out for the first time. the high immunomodulatory activity of P. agglomerans lPs suggests the possibility to use their oligosaccharide fragments in the development of conjugated vaccines against diseases caused by gram-negative bacteria.

Interaction of bacteriophage P1 with an epiphytic Pantoea agglomerans strain—the role of the interplay between various mobilome elements

“…The core oligosaccharide of P. agglomerans LPS is highly diversified. In the case of several strains described, it is similar to that of E. coli in that it contains glucose in the core oligosaccharide (Karamanos et al, 1992;Lerouge and Vanderleyden, 2002;Kohchi et al, 2006;Hashimoto et al, 2017;Varbanets et al, 2019;Bulyhina et al, 2020). Therefore, it was unsurprising that P1 adsorption to P. agglomerans L15 cells was possible and similar to that of E. coli K-12 cells concerning time and efficiency (Supplementary Figure 1).…”

“…Therefore, it was unsurprising that P1 adsorption to P. agglomerans L15 cells was possible and similar to that of E. coli K-12 cells concerning time and efficiency ( Supplementary Figure 1 ). Likely, not all P. agglomerans strains can adsorb P1, as not all contain glucose in their core LPS oligosaccharide (Varbanets et al, 2019 ). However, P1 requires only one receptor-binding protein (tail fiber) to adsorb to a host cell and inject its DNA (Liu et al, 2011 ; Lam et al, 2021 ).…”