Сиквенсные варианты онкогена<i>LMP1</i>у больных опухолями полости рта, ассоциированными и не ассоциированными с вирусом Эпштейна-Барр

Gurtsevitch, Vladimir; Яковлева, Л. С.; Щербак, Л. Н.; Гончарова, Е. В.; Smirnova, Kseniya V.; Дидук, С. В.; Кондратова, В. Н.; Максимович, Д. М.; Лихтенштейн, А. В.; Сенюта, Н. Б.

doi:10.7868/s0026898413050042

Cited by 3 publications

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“…Последующие углубленные исследования, включая серологические, позволили идентифицировать ВЭБ в качестве нового представителя семейства герпесвирусов. В 1979 году научное название изменили на Human herpesvirus 4, в 2016 году -ещѐ раз, для указания подсемейства, к которому вид относится, на Human gammaherpesvirus 4 [7,8,9].…”

Section: история открытия вируса эпштейна-баррunclassified

Современные Представления Об Этиологии Эпштейн – Барр Вирусной Инфекции

Нечукин,

Хрипченко,

Лидохова

2022

ТРНиО

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Герпесвирусные инфекции занимают лидирующие позиции в человеческой популяции. Особенность вируса Эпштейна–Барр (ВЭБ) заключается в его генетическом коде. Основную часть генома составляют участки, экспрессирующие регуляторные белки, которые позволяют реализовывать трехступенчатый жизненный цикл.

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Section: история открытия вируса эпштейна-баррunclassified

Современные Представления Об Этиологии Эпштейн – Барр Вирусной Инфекции

Нечукин,

Хрипченко,

Лидохова

2022

ТРНиО

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“…Информации о связи заболеваний с определенным типом ВЭБ недостаточно. В РФ изучение типов ВЭБ ограничивается единичными работами, посвященными только оценке распространенности ВЭБ-1 и ВЭБ-2 среди взрослого условно здорового населения [3,20].…”

Section: Introductionunclassified

Molecular genetic characterization of the epstein–Barr Virus: a relationship with the clinical features of pediatric infectious mononucleosis

Popkova,

Utkin,

Filatova

et al. 2023

Russian Journal of Infection and Immunity

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Introduction. Infectious mononucleosis (IM) is an high priority viral infection in children. Epstein-Barr virus (EBV) is the main etiological agent of IM. EBV is classified into two main types EBV-1 and EBV-2. In addition, different variants of the virus are isolated based on individual genes, among which the LMP-1 gene and the oncoprotein it encodes are the most well known. So far, the study of the clinical significance of EBV genetic diversity in EBV-IM in children in Russia has not been conducted. The aim of the study was to evaluate a relationship between EBV LMP-1 molecular genetic variants and clinical and laboratory manifestations of IM in children. Materials and methods. The material of the study was presented by blood leukocyte and saliva samples of children aged 117 years with EBV-IM (n = 69). A total of 132 EBV isolates were studied. For differential detection of EBV-1/EBV-2, we used a previously optimized one-round PCR variant with electrophoretic detection of amplification products in agarose gel. The nucleotide sequences of the C-terminal region of the LMP-1 gene were determined by Sanger sequencing followed by analysis of the obtained sequences using the MEGA X software. Multiple Factor Analysis was used to search for the relationship between LMP-1 variants and clinical and laboratory manifestations of IM (32 signs and 8 groups of signs). Results. It was established that only one type of virus, EBV-1, was identified in all children. At the same time, the severity of clinical manifestations of EBV-IM in children varied significantly (from 15.5 to 35.5 scores in total). Molecular genetic analysis of the sequences of the LMP-1 C-terminal region in Nizhny Novgorod region EBV isolates demonstrated a significant heterogeneity of the viral population, which was not limited only to their grouping according to known variants. According to the frequency of detection, B95-8 was the dominant variant of LMP-1 (60.66.0% of cases), other variants were less common (China 1, NC, Med and China 1+В95-8). It was found that EBV-IM proceeded more easily and with less severity of the intoxication syndrome in cases of infection with a virus having the molecular genetic profile of EBV-1/B95-8, in particular EBV-1/B95-8/E214D. Conversely, EBV-1/Med, as well as EBV-1/Med/L338S, EBV-1/Med/S229T, EBV-1/China 1/L338S and EBV-1/NC/S229T profiles were associated with more severe infection. Conclusion. For the first time, the influence of the genetic diversity of EBV on the clinical manifestations of IM in children was revealed. In the context of the tasks to be solved in this study, it is necessary to conduct a larger-scale and systemic studies in different territories of Russia.

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Ancient variants of the Epstein–Barr virus (Herpesviridae, Lymphocryptovirus, HHV-4): hypotheses and facts

Smirnova¹,

Senyuta²,

Lubenskaya³

et al. 2020

Problems of Virology

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Introduction. Molecular studies have shown that viruses appeared in the early stages of the evolution of life. For millions of years, viruses have evolved by changing old and acquiring new sequences in their RNA or DNA. It is assumed that most viruses have common ancestors. Such an ancestor, an ancient strain, probably existed for Epstein-Barr virus (EBV) as well.Aim. To find out whether ancient strains of EBV persist in modern Russian ethnic groups today.Material and methods. The object of the study was the EBV LMP1oncogene, which is most suitable for molecular genetic analysis. LMP1 was amplified from the oral cavity washings obtained from representatives of two ancient ethnic groups of Russia - Tatars and Slavs. The LMP1 amplicons were sequenced in both directions; their nucleotide sequences translated into amino acid (LMP1) were evaluated using the classification suggested by Edwards et al. 1999. To establish genetic relationships between LMP1 variants, a phylogenetic tree was constructed by the neighbor-joining method using the MEGA software package.Results and discussion. Analysis of LMP1 sequences from washings of the Slavs oral cavity demonstrated the presence of LMP1 variants with varying degrees of transforming potential: B98.5/A, China1, Med-, and NC. The analysis of LMP1 sequences from washings of Tatar oral cavity also made it possible to identify oncoprotein variants such as B95.8/A, China1, Med-, as well as a group of variants out of classifications (LMP1-OK). An important finding was the identification of 7 variants of LMP1 from Tatars, designated as LMP1-TatK, that contained two unique deletions of 5 aa in codons 312-316 and 382-386, which were absent in the LMP1 variants from Slavs and from previously examined cancer patients and healthy individuals, as well as in sequences from open computer databases. The uniqueness of the LMP1-TatK variant is confirmed both by phylogenetic analysis of LMP1 sequences of Tatar origin and by the analysis of 11 aa repeats and 5 aa insertions in the C-terminal region of the oncoprotein. The morbidity and mortality rates from neoplasms, including EBV-associated pathologies, did not differ significantly between two studied ethnic groups infected with different EBV strains.Conclusion. The data obtained allowed us to suggest that: 1) LMP1-TatK could be refered to an evolutionarily ancient EBV strain that persists among Tatars and; 2) LMP1-TatK belongs to the so-called “Volga” EBV virus strain, the common strain among the population of the Volga region. Extended studies of EBV isolates from residents of this region may probably shed the light on the origin of LMP1-TatK.

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Сиквенсные варианты онкогенаLMP1у больных опухолями полости рта, ассоциированными и не ассоциированными с вирусом Эпштейна-Барр

Cited by 3 publications

References 0 publications

Современные Представления Об Этиологии Эпштейн – Барр Вирусной Инфекции

Современные Представления Об Этиологии Эпштейн – Барр Вирусной Инфекции

Molecular genetic characterization of the epstein–Barr Virus: a relationship with the clinical features of pediatric infectious mononucleosis

Ancient variants of the Epstein–Barr virus (Herpesviridae, Lymphocryptovirus, HHV-4): hypotheses and facts

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