Характеристика Морфологічних Ознак Та Фізіологічних Властивостей  Штамів Сальмонел, Ізольованих Від Птиці І Телят

Boiko, O.P.; Сень, О М; Boiko, Pavlo; Куртяк, Б М; Pundyak, T.O.; Sobko, G. V.

doi:10.15421/nvlvet7826

Cited by 3 publications

(3 citation statements)

References 1 publication

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“…It has been found that avian salmonellosis vaccines should contain protective antigens that would stimulate the formation of protective antibodies against the above-mentioned salmonella serotypes (Trockyi, 2012). There were also found patterns in the manifestation of virulence and antigenic properties of the studied strains of Salmonella, namely, the higher the virulence of the strain, the higher its antigenic activity (Boiko et al, 2017). To develop vaccines against salmonellosis of poultry, socially important strains of Salmonella which most often cause food poisoning are chosen, such as S. typhimurium, S. enteritidis and S. gallinarum (Nair et al, 2018;Cao et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity and duration of immunity of the polyvalent vaccine against chicken salmonellosis

Сень

Saliy

Mazurkevych

et al. 2020

Regul. Mech. Biosyst.

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Poultry salmonellosis causes serious economic damage and must be prevented by disinfection, zoohygienic measures or by vaccination. To develop a new polyvalent vaccine against poultry salmonellosis, we used bacterial strains of Salmonella enteritidis, S. typhimurium and S. gallinarum. Antigenic and immunogenic efficacy of the vaccine was tested on specific-pathogen free chickens, which were divided into five groups of 10 birds in each group and were vaccinated intramuscularly at 8 and 12 weeks: group A (non-immunized control), group B (S. enteritidis mono-vaccine), group C (S. typhimurium mono-vaccine), group D (S. gallinarum mono-vaccine) and group E (trivalent vaccine Polimun Salmo). None of the immunized birds showed such adverse reactions as abnormal behaviour, mortality or signs of anorexia, depression or diarrhea. Two weeks after the revaccination, 5 birds in each group were challenged by watering 3 cm3 of working suspensions of S. gallinarum, S. typhimurium and S. enteritidis control strains at a concentration of 1 × 109 CFU. 72 h after the challenge, faeces were collected from all chickens in each group to identify Salmonella excretion with faeces, and the chickens were euthanized. Significant protection against the virulent challenge was observed in all immunized groups based on mortality and post-mortem lesions compared with the non-immunized control group. Blood samples were selected weekly from 5 chickens of each group for 184 days. The antigenic efficacy of the vaccines was studied by reaction of haemagglutination in the obtained serum. The potent antigen-specific response to lymphocyte activation found in all immunized groups indicated the induction of immune responses. Overall, the results showed that persistent immunity is formed in 4 weeks after the revaccination and lasts for a productive period. Immune response of chickens on day 184 after vaccination with Polimun Salmo was 1: 647, indicating that the developed polyvalent vaccine against common serovars of S. enterica in poultry is effective and immunogenic and can be further used in field studies.

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Section: Discussionmentioning

confidence: 99%

Immunogenicity and duration of immunity of the polyvalent vaccine against chicken salmonellosis

Сень

Saliy

Mazurkevych

et al. 2020

Regul. Mech. Biosyst.

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“…Зважаючи на це, нами підібрано виробничоконтрольні штами сальмонел (Boiko et al, 2017), розроблено і запропоновано низку вакцинних препаратів (моно-, бі-і тривалентних, водних і емульсованих), в результаті яких було встановлено, що найефективнішою проти сальмонельозу птиці виявилася бівалентна інактивована емульсована вакцина, виготовлена із S. typhimurium і S. enteritidis -штамів, які були виділені від птиці і виявили високі антигенні та проективні властивості . Вакцина пройшла відповідні лабораторні випробування за показниками безпеки та якості.…”

Section: результати та їх обговоренняunclassified

Production testing of antigenicity and immunogenicity of bivalent inactivated vaccine salmonellosis vaccine

Boiko¹,

Сень

Куртяк

et al. 2019

SMLNUVMB

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Scientists of both human and veterinary medicine combine their potential to develop new or improve old instruments in order to rein the problem of foodborne salmonellosis in Ukraine. According to the experience of European poultry industry, the most effective measure for the prevention of avian salmonellosis is total vaccination against salmonellosis of laying hens and breeding birds. In our country, the epizootic situation of salmonellosis of animals and poultry is consistently favorable. At the same moment nearly 90% of foods born Salmonella outbreaks are of poultry and egg products origin. In other words the source of major source of Salmonella agent is avian origin. Currently no vaccines of native origin have been registered in Ukraine. Although there have been numerous attempts to develop a vaccine against avian salmonellosis. The purpose of our work is to evaluate the antigenicity and immunogenicity of the two experimental series of bivalent inactivated emulsified vaccine against avian salmonellosis in production conditions. During the examination of the vaccine in the poultry farm it was found that for 21 days after the re-introduction of the vaccine, the titers of antibodies to the mono-antigens S. Typhimurium, Enteritidis and Gallinarum in the Agglutination Test (AT) and Indirect Hemagglutination Test (IH) were: 1 : 640–1280 to 1 : 2560–5120 respectively. This indicates high antigenicity of the vaccine. No significant difference between the levels of antibodies to Typhimurium and Enteritidis antigens was detected neither in AT nor IH. At the same time the levels of antibodies to mono-antigen Gallinarum were markedly lower in both reactions (1 : 160–1 : 320 – in AT and 1 : 320–1 : 1280 – in IH), but high enough to indicate that the vaccine creates a tense cross-humoral immunity to Salmonella surface antigens of Gallinarum serovar. The results of study of immunogenicity of the vaccine show that the vaccine is highly immunogenic. It means that after control infection of vaccinated hens none of tested Salmonella strains (S. Typhimurium and S. Enteritidis) were isolated from any organs, whereas in the control non-vaccinated group of birds both Salmonella test-strains were isolated from all organs. The obtained results provide a basis for further phases of the vaccine estimating followed by its registration in the prescribed manner.

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“…В більшості випадків в їхній основі лежать інфекційні захворювання переважно бактерійного походження. Водночас існує багато факторів як біотичних, так і абіотичних, які виступають пусковим механізмом та значно впливають на подальший розвиток цих захворювань, а також ефективність методів та засобів їх профілактики і лікування (Kurtyak et al, 2017;Boiko et al, 2017;Levkivska et al, 2017;Kysera et al, 2018;Gutyj et al, 2018).…”

Section: вступunclassified

The study of essential oil on antimicrobial activity and their composition towards microorganism

Kovalenko¹,

Napnenko²,

Chornyi

et al. 2018

SMLNUVMB

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In the course of the research, the microorganisms that often caused disease for animals were used, according to the literature, were genetically predisposed to resistance to traditional antibiotics and needed to find new effective means to combat them. It was used different antibiotic substances benzalkonium chloride, silver nanoparticles, and promising in this direction, essential oils of plants: fir, eucalyptus. The article provides information on the selection and study of the efficacy of substances, components with essential oils necessary for the development of complex bactericidal agents and the study of their antimicrobial activity against microorganisms. In determining the antimicrobial activity of essential oils, test cultures were used: Microccoccus luteus ATCC9341, Microccoccus lisogenicus, B. subtilis AATCC6633, B.cereus ATCC6633, S. aureus 209-P, S. zooepidemicus, Clebsiella spp, Salmonella cholerae suis, Pasterella multocida from the museum of State Scientific and Control Institute of Biotechnology and strains of microorganisms. The determination of fungicidal acity was performed on microscopic molds of Penicillium citrinum, Penicillium urticae, Aspergillus flavus, Aspergillus ochraceus. In experiments with Staphilococcus aureus, Streptococcus zooepidemicus, Pasteurella multocida, the antibiotic action of essential oils of eucalyptus and puff was confirmed and the possibility of its enhancement by the addition of quaternary ammonium compounds was confirmed. According to the results, the use as an alternative to antibiotics and disinfectants of preparations based on vegetable essential oils in the form of aerosols not only improves the number of animals from respiratory infections of bacterial origin, but also significantly improves the ecological conditions of the work of personnel in livestock buildings, promotes treatment and prevention respiratory diseases. The investigations have proved the expediency of using vegetable essential oils for the design of highly effective drugs and the possibility of enhancing their antimicrobial activity by quaternary ammonium compounds and silver nanoparticles, as well as a complex preparation «Asept» for aerosol application in the presence of animals.

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Характеристика Морфологічних Ознак Та Фізіологічних Властивостей Штамів Сальмонел, Ізольованих Від Птиці І Телят

Cited by 3 publications

References 1 publication

Immunogenicity and duration of immunity of the polyvalent vaccine against chicken salmonellosis

Immunogenicity and duration of immunity of the polyvalent vaccine against chicken salmonellosis

Production testing of antigenicity and immunogenicity of bivalent inactivated vaccine salmonellosis vaccine

The study of essential oil on antimicrobial activity and their composition towards microorganism

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