The new localities of six charophyte species have been found in Upper Volga basin based on our field sampling mostly during last years. It also allowed updating available data for two localities previously known. The genus Tolypella represented with T. prolifera has been reported in the region for the first time. The recent presence of Nitella syncarpa in Upper Volga basin as well as presence of N. flexilis in Tver Region have been confirmed. The last species was reported earlier without voucher specimens. The distributional data for Chara virgata in Tver Region were significantly updated. The data summarized for number of species localities in Moscow, Tver, Nizhniy Novgorod and Yaroslavl regions have been reported. They confirm particular species conservation status suggested earlier.