Цитотоксичность Трусопта И Дорзолана Соло На Первичной Культуре Эндотелиальных Клеток Роговицы Человека

Суббот, Анастасия Михайловна; Fisenko, N V; Труфанов, Сергей Владимирович

doi:10.30906/0869-2092-2020-83-7-27-31

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…При изучении ингибиторов карбоангидразы доказано, что бесконсервантный дорзоламид обладает наименьшей цитотоксичностью для культуры человеческих эндотелиальных клеток роговицы в сравнении с кон-сервантными препаратами тимолол, трусопт, траватан, ксалатан [48,49].…”

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Prerequisites to Use Preservative-Free Drugs in Medication Therapy of Glaucoma

et al. 2021

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Purpose — to analyze the literature data on the benefits of preservative-free medication therapy for glaucoma.Materials and methods. 482 articles in the Pubmed database published from 1975 to 2020 were analyzed using the terms: “glaucoma”, “preservatives”, “preservative-free therapy”, “benzalkonium chloride”. The review includes 78 articles. Inclusion criteria: studies that examined the effect of local antihypertensive therapy on the condition of the ocular surface in comparison with preservative-free drugs.Discussion. BAC is the most common preservative used in ophthalmology, accounting for 70 % of eye drop formulations. But it has a pronounced toxic effect on the ocular surface, so other classes of preservatives have been developed. These include polyquaternium-1 detergent; oxidizing preservatives — stabilized oxychlorocomplex and sodium perborate; ionic buffer preservative. However, most of the publications are devoted to BAC, as it is considered the most toxic and, at the same time, the most commonly used preservative. There is a large number of switching studies from preservative therapy to preservative-free, in which almost all groups of drugs used for the treatment of glaucoma were studied, but the use of antihypertensive drugs is associated not only with the action of preservatives, but also with the active substance, excipients and buffers. However, most switching studies demonstrate the benefits of preservative-free drugs. Other studies compared preservative and preservative-free drugs with the same active ingredients, in which a comparable hypotensive effect and safety profile was revealed, which indicates that BAC is not required for adequate penetration of the active substance to the point of application. At the same time, statistically significant advantages in the tolerability of preservativefree therapy were also revealed.Conclusion. Glaucoma preservative therapy has a toxic effect on the ocular surface, which leads to a decrease in the quality of life and compliance. Switching to preservative-free drugs can help reduce iatrogenic complications during long-term treatment of patients with glaucoma

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Section: Discussionunclassified

Prerequisites to Use Preservative-Free Drugs in Medication Therapy of Glaucoma

et al. 2021

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“…PRP по сравнению с плазмой крови, не содержащей тромбоциты и растворимые факторы тромбоцитов, и сывороткой крови, содержащей естественный (в 3-5 раза ниже) уровень растворимых факторов тромбоцитов. В работах авторов с использованием других экспериментальных исследований были получены аналогичные данные[26,29].ЗАКЛЮЧЕНИЕДанное исследование показало, что внутрисуставное введение модифицированного ПОРФТ/PRP приводило к выраженному терапевтическому эффекту, проявлявшемуся улучшением морфофункционального состояния гиалинового хряща (MANKIN score -2 балла) на шестые сутки, в сравнении с плазмой и сывороткой.…”

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Pathomorphologic evaluation of intra-articular injections of soluble platelet-rich plasma for treatment of experimental osteoarthritis

Tretyakov,

Zinovkin,

Karpenko

et al. 2024

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Introduction Non-surgical treatment of osteoarthritis is aimed at managing joint degeneration and inflammation to prolong the life of the original joint and delay total joint replacement. The objective was to pathomorphologically substantiate preclinical effectiveness of PRP in OA using comparative analysis of depleted plasma and serum.Material and methods The experiment was performed in 120 Wistar rats, divided into 4 groups. Osteoarthritis was simulated using an original method. Knee joint injection given to the animals after skin dissection under inhalation anesthesia and visual control two weeks later contained 0.05 ml PRP in group 1, 0.05 ml plasma in groups 2 and 0.05 ml blood serum in groups 3. The same volume of physiological saline solution was used for the injections produced for control animals. Injections were administered three times at 2-week intervals. Animals were sacrificed in groups of 10 at 2 weeks of each injection.Results The median MANKIN value scored 2.0 (1.0; 2.0) in group 1, 6.0 (5.0; 7.0) in group 2 and 7.0 (6.0; 7.0) in group 3 at 6 weeks. The median MANKIN value scored 7.5 (7.0, 8.0) in the control group. Statistically significant differences were determined between the groups at p < 0.001.Discussion Literature data on preclinical evaluation of the effectiveness of PRP therapy in biological models of OA are controversial. An original, low-traumatic functional method was used for simulating knee OA to reproduce major pathogenetic mechanisms in rats.Conclusion The findings suggested a pronounced therapeutic effect with improved morphofunctional features of the hyaline cartilage and MANKIN score of 2 at 6 days of intra-articular administration of modified PRP as compared with plasma and serum.

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