تقييم النشاط المضاد للتقرح والمضاد للأكسدة للشيتوزان فى الفئران المصابة بالقرحة الناجمة عن الإيثانول

Abstract

Chitosan is a polysaccharide of natural origin. It has been applied in many fields, such as food and medicine, and it has antioxidant properties, so the present study was carried out to evaluate the anti-ulcerogenic and anti-oxidative activity of chitosan in rats. Thirty adult male albino rats weighing (170g ±5 g) were randomly divided into five groups (6 rats each); the first and second groups were fed a standard diet. The third, fourth, and fifth groups were fed a diet containing 2.5, 5, and 7.5% chitosan powder, respectively. At the end of the experimental period (30 days), the rats were deprived of food for 24 h with free access to water. The second, third, fourth, and fifth groups were given a single oral dose of ethyl alcohol at 10 ml/kg body weight to induce gastric ulceration. In contrast, the negative control group received a single oral dose of saline (0.9%, w/v). After 2 hours of administration of ethyl alcohol, stomachs were ligated around cardiac and pyloric, and gastric juice was collected. Results revealed that rats pretreated with chitosan led to a decrease in ulcer score from 11 to 5, an ulcer index of 1100 to 500, an ulceration% from 86 to 17.2, and an increase in the preventive index from 14 to 82.8, and activity of antiperoxidative enzymes compared with positive control rats. Rats supplemented with 7.5% chitosan have the most potent gastric cytoprotective and ulcer healing-promoting. In conclusion, administering chitosan as a protective agent prevents stomach ulcers in rats.