三个蛋白精氨酸甲基转移酶基因在爪蛙胚胎发育中表达图式的研究

Wang, Chengdong; Guo, Xiaofang; Wong, Thomas Chi Bun; Wang, Hui; Qi, Xufeng; Cai, Dongqing; Deng, Yi; Zhao, Hui

doi:10.24272/j.issn.2095-8137.2018.064

Cited by 4 publications

References 23 publications

(32 reference statements)

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ZSWIM4 regulates embryonic patterning and BMP signaling by promoting nuclear Smad1 degradation

Wang,

Liu,

Zeng

et al. 2024

EMBO Rep

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The dorsoventral gradient of BMP signaling plays an essential role in embryonic patterning. Zinc Finger SWIM-Type Containing 4 (zswim4) is expressed in the Spemann-Mangold organizer at the onset of Xenopus gastrulation and is then enriched in the developing neuroectoderm at the mid-gastrula stages. Knockdown or knockout of zswim4 causes ventralization. Overexpression of zswim4 decreases, whereas knockdown of zswim4 increases the expression levels of ventrolateral mesoderm marker genes. Mechanistically, ZSWIM4 attenuates the BMP signal by reducing the protein stability of SMAD1 in the nucleus. Stable isotope labeling by amino acids in cell culture (SILAC) identifies Elongin B (ELOB) and Elongin C (ELOC) as the interaction partners of ZSWIM4. Accordingly, ZSWIM4 forms a complex with the Cul2-RING ubiquitin ligase and ELOB and ELOC, promoting the ubiquitination and degradation of SMAD1 in the nucleus. Our study identifies a novel mechanism that restricts BMP signaling in the nucleus.

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ZSWIM4 regulates embryonic patterning and BMP signaling by promoting nuclear Smad1 degradation

Wang,

Liu,

Zeng

et al. 2024

EMBO Rep

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Modification of maternally defined H3K4me3 regulates the inviability of interspecific Xenopus hybrids

et al. 2023

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Increasing evidence suggests that interspecific hybridization is crucial to speciation. However, chromatin incompatibility during interspecific hybridization often renders this process. Genomic imbalances such as chromosomal DNA loss and rearrangements leading to infertility have been commonly noted in hybrids. The mechanism underlying reproductive isolation of interspecific hybridization remains elusive. Here, we identified that modification of maternally defined H3K4me3 in Xenopus laevis and Xenopus tropicalis hybrids determines the different fates of the two types of hybrids as te×ls with developmental arrest and viable le×ts. Transcriptomics highlighted that the P53 pathway was overactivated, and the Wnt signaling pathway was suppressed in te×ls hybrids. Moreover, the lack of maternal H3K4me3 in te×ls disturbed the balance of gene expression between the L and S subgenomes in this hybrid. Attenuation of p53 can postpone the arrested development of te×ls. Our study suggests an additional model of reproductive isolation based on modifications of maternally defined H3K4me3.

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Kindlin2 regulates neural crest specification via integrin-independent regulation of the FGF signaling pathway

Wang

Long

et al. 2021

Development

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The focal adhesion protein Kindlin2 is essential for integrin activation, a process that is fundamental to cell-extracellular matrix adhesion. Kindlin2 is widely expressed in mouse embryos, and its absence causes lethality at the peri-implantation stage due to the failure to trigger integrin activation. The function of kindlin2 during embryogenesis has not yet been fully elucidated as a result of this early embryonic lethality. Here, we showed that kindlin2 is essential for neural crest (NC) formation in Xenopus embryos. Loss-of-function assays performed with kindlin2-specific morpholino antisense oligos (MOs) or clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 techniques in Xenopus embryos severely inhibit the specification of NC. Moreover, integrin-binding deficient mutants of Kindlin2 rescued the phenotype caused by loss of kindlin2, suggesting that the function of kindlin2 during NC specification is independent of integrins. Mechanistically, we found that Kindlin2 regulates the fibroblast growth factor (FGF) pathway, and promotes the stability of FGF receptor 1. Our study reveals a novel function of Kindlin2 in regulating FGF signaling pathway and provides mechanistic insights into the function of Kindlin2 during NC specification.

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三个蛋白精氨酸甲基转移酶基因在爪蛙胚胎发育中表达图式的研究

Cited by 4 publications

References 23 publications

ZSWIM4 regulates embryonic patterning and BMP signaling by promoting nuclear Smad1 degradation

ZSWIM4 regulates embryonic patterning and BMP signaling by promoting nuclear Smad1 degradation

Modification of maternally defined H3K4me3 regulates the inviability of interspecific Xenopus hybrids

Kindlin2 regulates neural crest specification via integrin-independent regulation of the FGF signaling pathway

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