Theoretical and experimental investigations of native bone are carried out to understand relationships between its hierarchical organization and local electronic and atomic structure of the mineralized phase. The 3D superlattice model of a coplanar assembly of the hydroxyapatite (HAP) nanocrystallites separated by the hydrated nanolayers is introduced to account the interplay of short-, long- and super-range order parameters in bone tissue. The model is applied to (i) predict and rationalize the HAP-to-bone spectral changes in the electronic structure and (ii) describe the mechanisms ensuring the link of the hierarchical organization with the electronic structure of the mineralized phase in bone. To check the predictions the near-edge x-ray absorption fine structure (NEXAFS) at the Ca 2p, P 2p and O 1s thresholds is measured for native bone and compared with NEXAFS for reference compounds. The NEXAFS analysis has demonstrated the essential hierarchy induced HAP-to-bone red shifts of the Ca and P 2p-to-valence transitions. The lowest O 1s excitation line at 532.2 eV in bone is assigned with superposition of core transitions in the hydroxide OH(HO) anions, Ca(HO) cations, the carboxyl groups inside the collagen and [PO] and [PO] anions with unsaturated P-O bonds.

The hundreds thousands total hip arthroplasties (THA) annually  performed around the world with the number of revision procedures  estimated around 10%. Dual-energy X-ray absorptiometry (DEXA)  allows to control projectional bone mineral density (BMD) around hip stem dynamically with minor radiation exposure and in a cost-effective way. In routine practice there is a monthly interval between investigations that does not allow to evaluate activity and trends in  short weekly oscillations of bone metabolism in peri-prothetic areas.  Projectional BMD oscillations in peri-prosthetic zone after total hip  arthroplasty in chrono-biological manner evalua ted for the first  time. Therefore chronobiologic approach opens new opportunities for prognosis of tendencies in structural and functional skeleton  reorganization in peri-prothetic zone after THA but there is need in  further research that could give more insight.