The current study included the effect of gender, age and date of collection on the Pistacia vera gum growing in the Nineveh province. The study included the effect of gender (female, male), three ages (16, 23, 35) and the date of collection included three dates (July, August and September). Gum collected from each trees, the chemical fractionation of the gum was carried out as essential oils and polymer, also acid group types (A, B, C). The results showed the significant effect of the species of the tree, age and date of collection on the amount of gum, where the moral superiority of the gender of the male (23.06 g) on the female gender (19.34 g) and the increase in the quantity with age to28.95 g. At the age of 35 years, August was the best time for collection (25.0 g). Significant effects of age and date of collection were found on the percentage of essential oils, the highest percentage was at 16 years (15.8) and the percentage of essential oils in July and August were (15.2 and 15.1). The results showed the high significant effect of the age and the time of collection on the polymer ratio. The highest ratio 51.99% was at the age of 35 years and 49.71% for September. The proportion of polymer in the gender of the male was (49.47%).