This study was begin from January to December 2020 , to learn where there breast cancer and toxoplasma infection are related By used immunology test results . 2020, to know the association between Toxoplasma infection and breast cancer .by examine immunological test.32 women who had tumors were the subjects of a study conducted at the Al-Sadder Teaching Hospital in Al-Najaf Al-Ashraf , with breast tumor symptom . the mean age of women from 35-45 years ago . X-ray was done at first and sonar to detected the site of tumor and size . Biopsy was taken and sent to the Al- kafeel histopathological laboratory for making block and stain with hemaxlin and eoxin typical Histological change and ductal cancer show with irregular shape of duct mammary gland ,by use H & E method , serological was applied for toxoplasma appear in Five women is positive test for Toxoplasma AB by used special kit . the rest women consider control group . this study was find significant Toxoplasma associated with breast cancer , the case record at first time in Al-Najaf government.