A whole group of polymorphisms of genes involved in the formation of the epidermal barrier, immune responses, and their regulation is important in the formation of atopic phenotype. The purpose of the study is to determine the relationship of polymorphisms of genes of Toll-like receptors TLR2 and TLR4 with clinical and immunological parameters in atopic dermatitis patients in a “case-control” study. Polymorphisms of genes TLR2 (p.Arg753Gln) and TLR4 (Asp299Gly) were detected by PCR. Parameters of the state of innate and adaptive immunity were assessed by the level of local production of sIgA, cytokine profile of blood serum for IL-4, IL-10, and IFN-γ. Biological samples from 50 people with allergic pathology, aged 4.5 to 35 years, and 100 healthy individuals (controls) were analyzed. Observed dysregulation of cytokine production (IL-4, IL-10) in patients with heterozygous polymorphic genotypes probably reflects an imbalance of Th1/Th2/Th17 regulation of immune system response in these individuals.

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Aim. To assess the ability to form biofilms by clinical strains of the yeast Candida albicans isolated from patients with atopic dermatitis in exacerbation and remission stages under the effect of Fusarium solani micromycete and its absence. Methods. The study included 70 strains of C. albicans and one strain of F. solani. Fungal biofilms formed according to the method of Ramage. The optical density of the biofilms measured using a micro plate reader at 620 nm. The effect of associated fungi on the biofilm-forming properties of C. albicans strains was studied by an extract from opportunistic F. solani fungi. Results. The greatest biofilm formation was observed in strains isolated at the remission stage. The strains isolated in the acute period were inferior to them in the ability to form biofilms (average values of film formation were 0.143 and 0.087, respectively). Co-cultivation of C. albicans strains with F. solani fungus extract stimulated biofilm formation of C. albicans strains at a concentration of 1:10. Conclusion. This study showed a possible synergism between C. albicans and F. solani in polymicrobial skin infections, because the products of the fungus F. solani increase one of the virulence factors of the fungus C. albicans; the possibility to assess of a stimulating effect of associated fungi on the virulence one of an agent of infectious disease process will allow predicting the disease severity.