Among ten of Aspergillus isolates were detected for pectin hydrolysis isolated from different Governorates in Egypt on pectin screening agar medium (PSA), four isolates of Aspergillus were observed with high of pectinase production by clear zone method which were 3.62, 3.70, 3.91, and 4.75mm for these isolates (F4, F5, F9 and F10). The isolate F9 of Aspergillus was selected for height pectinase production it was recorded the maximum zone of clearance (4.75mm) as enzyme activity. These isolate has been identified as Aspergillus flavus (MH237625.1) according to microscopic characteristics, and molecular biology by ITS. The optimization condition for production of pectinase enzyme such as; pH value, incubation temperature, incubation periods, carbon source, nitrogen source, and mineral salts by isolate F9 of Aspergillus flavus (F9 isolate). The productivity of enzymes was assayed in submerged fermentation condition. The maximum pectinase production was observed with pH 7.0 (32.64 U/ml), temperature 25°C (39.09 U/ml), incubation periods 5 days, (38.11 U/ml), maltose (30.79 U/ml), peptone (39.25 U/ml), and FeSO4 (37.41 U/ml) in the production medium.