Heat stress is one of the most crucial factors affecting crop growth and productivity worldwide. So, searching for a potent eco-friendly heat stress alleviator is the main issue nowadays. The current study was conducted to assess the ameliorative effects of 1.5 mM potassium silicate (K2SiO3, further only Si) or 1.66 mM silicon dioxide nanoparticles (SiNPs) on wheat (Triticum aestivum L.) seedlings exposed to heat stress (45 °C, 4 h). The observations show that Si or SiNPs treatments significantly restored the heat stress-provoked ultrastructural distortions of cellular organelles, particularly chloroplasts and the nucleus. Further, both Si and SiNPs enhanced the photosynthetic capacity as revealed by increments in the photochemical efficiency of photosystem II and the performance index as well as the content of photosynthetic pigments. A reduction in malondialdehyde accumulation in Si and SiNPs treated plants was positively related to their membrane stability index. The reverse transcription PCR analysis showed that Si treatment but not SiNP treatment stimulated the overexpressions of both Triticum aestivum plasma membrane intrinsic protein (TaPIP1) and Triticum aestivum nodulin 26-like intrinsic protein (TaNIP2) aquaporin genes parallelly with an improvement in the relative water content. This investigation reveals that Si was more effective than SiNPs in restoring the heat stress injuries. To the best of our knowledge, this is the first investigation exploring the effects of Si and SiNPs in improving thermotolerance of wheat seedlings.
SUMMARYOver a 15-year period nearly 2000 young females were exposed to the ram at 6 to 9 months of age. These Clun Forest females were born between January and April in the Cockle Park experimental flock, and mated between October and December of the same year. On average nearly 42% of those present at lambing did not produce a lamb. Analyses are presented of the reproductive efficiency of these animals and of some of the possible causes of their failure to lamb. Observations in 10 of these years suggested that only 12% had not been mated and might therefore be assumed to be prepuberal. Age at first oestrus for those animals achieving puberty in their first autumn was inversely related to date of birth. The number which lambed increased by 1·1% per kg increase in live weight before mating.In 3 years simple nutritional and housing treatments were applied to groups of female lambs. Feeding a cereal supplement to animals at pasture before, during and after mating, and housing 1 to 2 weeks after mating, appeared to increase the percentage which lambed. It is suggested that the effect was to reduce the amount of early embryo death caused by nutritional and climatic stresses on the normal grazing animal.
Triacontanol (TRIA) role in improving growth, physiological activities and tolerance against abiotic stresses has been reported. Yet, the mechanism by which TRIA executes its effects remains elusive. This work therefore studied the possible role of TRIA exogenous application in counteracting the adverse effects of nickel (Ni) treated maize seedlings. Maize seedlings (15-day-old) were grown in washed sand irrigated with nutrient solution provided with 100 μM NiCl2. Two concentrations of TRIA (25 and 50 µM) were applied twice as a foliar spray for Ni-stressed seedlings. Shoot and root growth attributes, Ni content, and antioxidant defence systems of maize seedlings were determined. Ni treatment reduced the shoot and root length and biomass, causing necrosis of the old leaves,greater reduction was shown in the roots. The shoot and root length was negatively correlated with their Ni content, which was consistent with their content of H2O2, but not with their malondialdehyde (MDA) content. As the roots had the greatest Ni content, maximum peroxidase (PX) and glutathione reductase (GR) activity as well as the highest ascorbic acid (ASA) and reduced glutathione (GSH) content were observed in the roots. The Ni-induced deleterious effects were alleviated by foliar application of TRIA concentrations. Also, TRIA treatment minimized root Ni content, whereas it maintained the shoots unharmed by Ni. Such mitigative effects of TRIA are explained by its key role in enhancing antioxidant capacity (expressed as IC50), increased PX and ascorbate oxidase (AO) activity, GSH, and total phenolic contents.
SummarySixty-four weaned Awassi ewe lambs were used in the present study to estimate age at puberty and to investigate whether early breeding from such ewe lambs is possible. After weaning at 4 months old ewe lambs were divided at random into two experimental groups. One group, designated as moderate, was fed to attain 40 kg live weight at 12 months of age, while the other (high) was planned to reach 50 kg at the same age. Fertile rams were run with the ewe lambs at 6 months of age. Twenty ewe lambs, 10 from each treatment, were slaughtered and their ovaries were examined for the presence of corpora lutea and largest follicles.The overall age at first oestrus was found to be 293 days. Ewe lambs of the high nutritional treatment reached puberty earlier than those of the moderate one (274 v. 312 days). Likewise, single-born lambs reached puberty earlier than twin-born ones (273 v. 312 days). Treatment differences explained 16·5% while type of birth explained 16·3% of the total variance in age at first oestrus. Ewe lambs of the high nutritional treatment experienced more heats than those of the moderate one. Simple correlation coefficients between age at first oestrus and date of birth, daily gain and live weight at puberty were 0·21, 0·09 and 0·75, respectively. Results indicated that ovulation may not occur during the first and second oestrus. From 44 ewe lambs only 12, 10 from the moderate and 2 from the high treatment, lambed 13 viable lambs. Reasons for such a low conception rate (27·3%) are discussed.
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