Present study aimed to assess the bacterial counts and physicochemical profile of wastewater in some cities (Tanta, El mehala Elkobra, Mahlet Abo Ali, Sammanod and Mahlet Roh) at El Gharbia Governorate, Egypt during four seasons (summer, spring, winter and autumn). The current work was done in wastewater samples for determination of total bacterial counts. The microbiological studies of wastewater showed that the highest number of total bacteria counts were found on summer season at El mehala Elkobra. The physicochemical properties of wastewater were tested to evaluate its suitability for the intended purpose. The physical parameters of wastewater indicated that the color is grey to black and temperature 29ºC in summer at all cities except Mahlet Abo Ali and Mahlet Roh. Results of chemical parameters of the wastewater samples showed that slight variation between different sites and seasons in all wastewater samples. While, pH showed that season has significant role in the acidity by wet of rainy season. The highest turbidity values were recorded in El mehala Elkobera ( 11.4 NTU) in the spring season. In addition, the highest values of electrical conductivity, total hardness, total dissolved solids, total sulphate, ammonia and nitrate were recorded in summer season with different sities. On the basis of the above mentioned results, efforts should be made to avoid the environmental pollution with wastewater.