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Abstract:The interest in synthesising inorganic nanomaterials for biological applications has increased in recent years, especially for antibacterial purposes. In the present study, spherical and cube-shaped copper nanoparticles were synthesised by a chemical reduction method and their efficacy as antimicrobial agents against both Gram-negative (Escherichia coli) and Gram-positive (Enterococcus sp) organisms investigated. The nanoparticles were characterised using ultra-violet/visible spectroscopy, scanning electron microscopy, energydispersive spectroscopy and x-ray diffraction. Copper nanocubes were found to be more antimicrobial when compared with copper nanospheres and it is postulated that whilst both sets of nanoparticles have similar total surface areas, the different shapes have different active facets and surface energies, which may lead to differing bactericidal behaviour.