Aims/hypothesis Cerebral oedema complicating diabetic ketoacidosis (DKA) remains the major cause of morbidity and mortality in children with type 1 diabetes, but its aetiology remains unknown. Our objective was to determine the impact of baseline biochemical factors and of treatment-related variables on risk of the development of cerebral oedema in children with DKA. Materials and methods This was a national UK casecontrol study. Through the British Paediatric Surveillance Unit we identified 43 cases of cerebral oedema. Through a parallel reporting system, we also identified 2,940 episodes of DKA and selected 169 control subjects on the basis of comparable age, sex, numbers of new or known cases of diabetes and date of admission. Baseline biochemical data and treatment-related variables were extracted from the clinical notes of cases and control subjects. Results Allowing for differences in age, sex and new or known diabetes, cases were more acidotic at diagnosis of DKA (odds ratio [OR] for events in the least acidotic compared with the most acidotic tertile=0.02 [95% CI: 0.002-0.15], p<0.001). In addition, cases had higher potassium and urea levels at baseline. Calculated osmolality and baseline glucose were not significantly different. After allowing for severity of acidosis, insulin administration in the first hour .5], p=0.02) and volume of fluid administered over the first 4 h .97], p=0.01) were associated with risk. Low baseline plasma sodium and an elevated p a CO 2 also contributed to risk in the final regression model. Bicarbonate administration was not associated with increased risk of an event when corrected for acidosis. Conclusions/interpretation In this case-control study of DKA, baseline acidosis and abnormalities of sodium, potassium and urea concentrations were important predictors of risk of cerebral oedema. Additional risk factors identified were early administration of insulin and high volumes of fluid. These observations should be taken into account when designing treatment protocols.

In children with DKA, initial conscious level is closely related to pH and weakly to age, but not to BG or plasma sodium level. Thus cerebral function in DKA is related to severity of acidosis even when there is no evidence of cerebral edema.

In this study, simultaneous monitoring of indoor and outdoor Volatile Organic Compounds (VOCs) was carried out in a school, by both Radiello® cartridges and real-time monitors (Corvus, IonScience Ltd., Fowlmere UK). Moreover, an outdoor air quality assessment was performed with data from an air quality monitoring station (ARPA Puglia) located close to the school. In particular, VOCs, Benzene, Toluene, Ethylbenzene, and Xilenes concentrations, obtained by using Radiello® diffusive samplers, were monitored in two classrooms, two bathrooms, and outside of the school building for three weeks during winter 2019. Simultaneously, the Total VOC (TVOC) concentrations were measured by means of real-time monitors inside and outside the classroom in order to individuate the activation of sources during the sampling campaign days. The results evidence that indoor TVOC concentrations were bigger than those outdoors; this suggests the presence of indoor pollutant sources, both in the classrooms and the bathrooms. The results of our study can help the school management by the following recommendations: increasing ventilation in classrooms during school activities and after cleaning; involving students in discussions regarding the use of acrylic paints, permanent markers, perfumes and deodorants, and the limitation of smoking activities both in bathrooms and outdoor spaces.

Salt crystallization is one of the most dangerous forms of degradation affecting frescoes. This phenomenon can lead to cracking, flaking and detachment of the pictorial layer, ultimately ruining the work of art. However, the characterization of soluble salts via chemical analysis can be employed to determine the conditions of the artifact and establish the proper restoration and/or conservation strategies to be adopted. In this archaeometric study, a first-ever characterization of the soluble salts and related degradation phenomena on the frescoes by Saturnino Gatti in the church of San Panfilo in Villagrande di Tornimparte (L’Aquila) was carried out. Sampling was performed in areas with evident detachments, exfoliations and saline crystallization (efflorescences). Eleven samples of powder and fragments were taken from different panels of the fresco: nine were taken from Panels A, C, D and E and two from the top part of the vault. Chemical characterizations were performed using two analytical techniques: ion chromatography (IC) and attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR). Ion chromatography was used for the quantification of the main ions and to evaluate the presence of soluble salts, whereas infrared spectroscopy was used to characterize the mineralogical phases. The results show efflorescence consisting of newly formed gypsum and carbonate in samples taken closer to the ground. Furthermore, a good correlation between sodium and chloride ions was observed, indicating the presence of an efflorescence composed of newly formed sodium chloride. Capillary rise and infiltration were highlighted as the main sources of soluble salts. This information will be crucial in guiding future restoration or conservation operations.

The present study deals with the set-up and development of a new methodology to quantify the carbonaceous fraction (i.e., OC, organic carbon and EC, elemental carbon) in black crusts samples present on marble stone surfaces of historical buildings and monuments exposed to outdoor pollution. It is worth noting that OC and EC represent one of the main fractions of atmospheric particulate matter and, mainly EC, is responsible for the black colour of the crusts. The same method has been applied to resuspend known quantities of atmospheric particulate matter on marble mock-up specimens suitably realized to study the crusts’ formation process starting from known quantities of the precursors in the formation process, i.e., PM and SO2, and submitting them to an accelerated aging process.