Our earth releases several types of green house gases into the atmosphere viz., carbon dioxide, nitrous oxide, methane, chlorofluorocarbon etc. Rice is a prime food crop in the Asian countries which fulfil the food security. Methane is one among the greenhouse gases which usually emitted from low land rice field. Methanogen survive in the absence of oxygen and usually releases methane from irrigated rice ecosystem.  Methanotroph are aerobic group of organism that require minor amount of oxygen to survive. Rice ecosystem is the ideal ecosystem for the survival of methanogens and methanotrophs. With this background methanotrophs were isolated from four different location of cauvery delta zone. Cultures were confirmed through methane monooxidase activity. Methanotroph isolates were mass multiplied and bioinoculants were developed.