Mites have usually been identified by their morphological characteristics. However, morphological evidences are not always sufficient to distinguish between closely related species, leading taxonomists to consider additional ecological, biological and, more recently, molecular characteristics in this process. The molecular characterization of populations of Euseius citrifolius Denmark & Muma and Euseius concordis (Chant) was done by sequencing the internal transcribed spacers ITS1 and ITS2 using the P1 (5'-AGAGGAAGTAAAAGTCGTAACAAG-3)' and P2 (5'-ATATGCTTAAATTCAGGGGG-3') primers, located in 18S and 28S regions of the ribosomal DNA. The following populations were characterizate: E. citrifolius: Arroio do Meio-RS, Campinas-SP and Petrolina-PE; E. concordis: Arroio do Meio, Jaguariúna-SP, Pontes e Lacerda-MT, Petrolina and Viçosa-MG. The sequencing of ITS1 and ITS2 allowed the discrimination between the group of populations of E. citrifolius from E. concordis. The similarity between the sequences was more than 94%. Most of the variation between populations was observed more in ITS1 than in ITS2. The sequencing of ITS region helps the identification of phytoseiid species when the populations show citoplasmatic incompatibility and need additional information.
This study reports the occurrence of the phytophagous mite Tetranychus marianae McGregor, 1950 (Tetranychidae), in species of the genus Piper (P. hispidum Sw., P. marginatum Jacq., P. montealegreanum Yunk., P. peltatum L., P. reticulatum L., and P. tuberculatum Jacq.) (Piperaceae) and the predator mite Amblyseius tamatavensis Blommers, 1974 (Phytoseiidae), in species and cultivars of Piper nigrum L., from the Piperaceae collection of Embrapa Amazônia Oriental in Belém, Pará.
RESUMO Considerando a ocorrência do ácaro Brevipalpus obovatus (Donnadieu, 1875) (Acari, Tenuipalpidae) em cultivos de maracujazeiro (Passiflora edulis Sims f. flavicarpa Deg. - Passifloraceae) no Estado da Bahia, a biologia dessa espécie foi estudada para avaliar o desenvolvimento e a reprodução em folhas de maracujá amarelo. O estudo foi conduzido em laboratório a 25 ± 1° C, 70 ± 10% de umidade relativa e 12 horas de fotofase. O período de ovo a adulto foi de 19,11 ± 0,16 dias, com sobrevivência de 86,11%. A longevidade média das fêmeas foi de 37,69 ± 3,06 dias e a oviposição média diária de 1,29 ± 0,05 ovos/fêmea. A razão intrínseca de aumento (rm) foi de 0,064, a razão finita de aumento (λ) de 1,066 fêmeas/fêmea/dia, a duração média de uma geração (T) de 38,94 dias e a taxa líquida de reprodução (Ro) de 12,13. Os resultados indicam que o maracujazeiro amarelo é um hospedeiro favorável ao desenvolvimento e reprodução de B. obovatus.
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