SUMMARY This is the first report of human gastrointestinal arachidonate and prostanoids measured quantitatively by gas chromatography-mass spectrometry (GC-MS) The methods for gas chromatography-mass spectrometry (GC-MS) were as reported previously.' In brief, the dried extract obtained as described above was dissolved in dichloromethane. An aliquot was taken for GC-MS and further purified by LH20 column chromatography; non-polar impurities were eluted with dichloromethane, and the eicosanoids were eluted with methanol which was then evaporated. Deuterated standards were added, and each dried methanol extract was dissolved in doubledistilled water acidified to pH 3 with hydrochloric acid. Each solution was percolated through an Amberlite column which was then washed with distilled water, and the eicosanoids were eluted with 315 on 11 May 2018 by guest. Protected by copyright.

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