Acidogenic prepartum diets with negative dietary cation-anion difference (DCAD) induce compensated metabolic acidosis, which stimulates calcium (Ca) mobilization before calving and decreases clinical and subclinical hypocalcemia postpartum. This strategy is often combined with limiting dietary Ca concentrations, which historically has been used to mobilize Ca prepartum to prepare cows for lactation. Supplemental dietary Ca in combination with a negative DCAD formulation that does not reverse the effect of compensated metabolic acidosis may be beneficial. Our objective was to determine the effects of prepartum dietary strategies on dry matter intake (DMI), milk production, peripartal Ca status, and health during the transition period in multiparous Holstein cows (n = 81). Treatments during the last 28 d before calving were:(1) positive DCAD diet, +6 mEq/100 g of DM, target urine pH >7.5, low dietary Ca (0.40% DM; CON); (2) negative DCAD diet, −24 mEq/100 g of DM, target urine pH 5.5 to 6.0, low dietary Ca (0.40% DM; ND); or (3) negative DCAD diet, −24 mEq/100 g of DM, target urine pH 5.5 to 6.0, , high dietary Ca (2.0% DM; NDCA). Preplanned treatment contrasts were:(1) CON versus (ND and NDCA), and (2) ND versus NDCA. Individual DMI were recorded daily. Cows were milked 3 times daily, with individual DMI and milk yield summarized by week. Whole blood sampled at calving and 24 h, 48 h, and 4 d after calving was analyzed for ionized Ca concentration, and serum was analyzed for total Ca. Prepartum urine pH for cows fed ND or NDCA averaged 5.7, whereas cows fed CON remained >7.5. During the 3 wk before calving, cows fed CON had greater DMI than cows fed ND or NDCA, with NDCA greater than ND. Postpartum DMI (% of body weight) tended to be less for cows fed CON than for those fed ND or NDCA prepartum. Thresholds for subclinical hypocalcemia were ionized Ca <1.0 mM at 24 h, and total Ca ≤2.125 mM at 48 h after calving. On average, blood Ca for cows fed CON indicated subclinical hypocalcemia, whereas blood Ca for cows fed ND or NDCA was greater than subclinical hypocalcemia thresholds for both ionized Ca and total Ca. No milk production differences were detected. Cows fed CON had an elevated adverse health score (calculated by assigning numerical values to recorded health events) and tended to have an elevated somatic cell count during the fresh period compared with cows fed ND or NDCA. Overall, an acidogenic diet prepartum without or with high Ca improved postpartum Ca status and health. Supplementation of additional Ca to the acidogenic diet had little effect.

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As many as 20% of all assessed amphibian species are threatened with extinction, and captive breeding programs are becoming important components of conservation strategies for this taxon. For some species, exogenous hormone administration has been integrated into breeding protocols to improve propagation. However, most treatments are administered by an intraperitoneal injection that can be associated with some risks. The general goal of this study was to identify a non-invasive method of applying luteinizing hormone-releasing hormone (LHRH), which reliably induces sperm release in toads. Specific objectives were to 1) test the spermiation response after topical application of different LHRH doses to the abdominal seat region, 2) evaluate the effects of adding the absorption enhancers dimethyl sulfoxide (DMSO), acetone, and glyceryl monocaprylate (GMC) to the LHRH, 3) assess the spermiation response after oral delivery of LHRH in a mealworm vehicle, and 4) compare sperm characteristics and spermiation responses to treatments in two different toad species. Male American (n = 9) and Gulf Coast (n = 7) toads were rotated systematically through a series of treatments. Urine was collected and evaluated for the presence of sperm at 0, 3, 7, 12, and 24 hours post-treatment. There were no statistical differences in spermiation induction or sperm characteristics between American and Gulf Coast toads after the treatments. Oral administration of 100 &mgr;g LHRH was occasionally successful in inducing spermiation, but results appeared largely unreliable. Ventral dermal application of 100 or 10 &mgr;g LHRH in 40% DMSO were more effective (P < 0.05) at inducing spermiation compared with the other treatments tested, eliciting sperm release in more than 70% of toads tested. In breeding programs for rare and/or fragile anurans, these non-invasive methods of exogenous hormone administration might be preferred over intraperitoneal injections. Zoo Biol 20:63-74, 2001. Copyright 2001 Wiley-Liss, Inc.

The goal of this study was to examine the ability of a commercially available feed additive (OmniGen-AF) to reduce mammary infections caused by a single strain of mastitic pathogens (Streptococcus uberis, Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae) and to examine the effects of the additive on markers of mammary immunity. Four experiments were completed using a murine model of bovine mastitis. Infection progression was examined using Sybr-green-and TaqMan-based quantitative PCR assays of 16S ribosomal DNA. Infection of the mammary gland with all pathogens caused rapid (24 to 48 h) appearance of pathogen DNA in mammary tissue. Provision of the feed additive for 2 weeks before infection significantly (P , 0.05) reduced the extent of pathogen DNA accumulation in models of S. uberis, E. coli and S. aureus infection. The additive was ineffective in reducing mammary infections caused by K. pneumoniae. We examined mechanisms of action of the additive through assessment of mammary concentrations of mammary myeloperoxidase (MPO), major histocompatibility complex 2 class II (MHC) and macrophage inflammatory protein-1a (MIP) messenger RNA (mRNA) concentrations and by examining serum complement C3 concentration. Infection of the mammary gland increased concentrations of MPO and MHC mRNAs (P , 0.05). Ability of the pathogen to elicit changes in mammary MPO and MHC gene expression was enhanced by the provision of the additive for 2 weeks before infection. These data imply that the additive increased the mammary inflammatory response and increased antigen presentation during a mammary infection. Value of the additive in preventing mastitis in cattle awaits additional studies using a bovine model and further evaluation of additional strains of the pathogens used in this study.

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