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The bees of dark forest species (Apis mellifera mellifera L.) are winter-hardy. These bees are able to effectively use a short honey harvest. Introgression of southern bee species in the Russia affects ro the economic quality of local northern species of bees. Introgression of species reduces their immunity, thereby It was enhancing susceptibility bees to infection with ectoparasites, as well as distribution of various viral diseases. Varroa destructor mite infestations on bees may predispose to infection by viruses. We have presented data about population differentiation of bees of dark forest species in Udmurtia, which were obtained based on the study using PCR method, morphometric parameters (cubital index of wing) and estimation of variability in intergenic locus of the CO-I-CO-II of mtDNA. Based on the analysis of the mtDNA the hybridization degree in samples of bees from Udmurtia 1UD_S and 5UD_S (maternal line) was 15.4 and 60.0%, respectively. Sample 2D_S-4UD_S meet the standards of dark forest bee species. For all analyzed samples (1UD_S-5UD_S) obtained average rating of cubital index> 0.55. These data confirmed the high probability of belonging of bees of sample data to the dark forest bee species. However, the variability analysis (according to the length of the intergenic locus CO-I-CO-II) with Southern species hybridization was observed only in samples 2UD_S-4UD_S, whereas in the other samples 1UD_S 5UD_S hybridization has occurred. This indicates a greater degree of reliability of molecular techniques in the evaluation of the degree of hybridization when samples of bees were studied. It's interesting that in one of the samples 2UD_S we founded a heteroplasmy PQ/P3*Q. In bees exposed to Varroa destructor defeat was detected deformed wing virus (DWV) (23,3% of families), chronic bee paralysis virus (CBPV) and sacbrood bee virus (SBV) (13, 3%).

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