The effect upon nodulation of lime-pelleting inoculated lupin and serradella seed has been examined in four localities and three growing seasons. Altogether, three adhesive solutions, four bacterial strains, and three leguminous species have been used in the experiments. The lime coat has markedly reduced the nodulation of these plants in all cases. Attention is drawn to the value of certain adhesives in maintaining higher numbers of viable bacteria on inoculated seed.

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ane HCl buffer (pH 8.0), 10.0 Amoles of MgCl2, and 0.5 ml of crude extract (3.1 mg of protein), were incubated at 37 C for 4 hr under anaerobic conditions with the following additions: (A) 13.0 ,moles of sodium glyoxylate and 12.0 ,umoles of propionyl-CoA; (B) 20.0 ,umoles of disodium ,B-methylmalate, 10 ,umoles of adenosine triphosphate, and 5 ,umoles of CoA-SH; (C) 10 ,moles of 3-methylmalyl-CoA. Propionyl-CoA was prepared according to the method of Simon and Shemin (J. Am. Chem. Soc. 75:2520, 1953) and f3-methylmalyl-CoA by the method used by Trans and Brody (J. Am. Chem. Soc. 82:2972, 1960) for the synthesis of methylmalonyl-CoA. f-Methylmalate was prepared according to the method of Scherp (J. Am. Chem. Soc. 68:912, 1946). The acyl-CoA derivatives were assayed by the hydroxamate method of Lipmann and Tuttle (J. Biol. Chem. 159:21, 1945). Protein was determined by the method of Warburg and Christian (Biochem. Z. 310:384,

The effectiveness of several isolates of nodule bacteria was tested on two species of serradella Ornithopus sativus Brot. and O. compressus L. analysis based on green top weights showed isolate S3 to be superior to the other isolates tested and also to the lupin strain W72, which is the only commercially available strain suitable for inoculation of serradella.