Poster abstracts the corpus callosum. Genetic amniocentesis was performed. All cells from two independent cultures showed an abnormal female karyotype with an inverted duplication of the short arm of chromosome 5 involving region 5p;13 to 5p;15.2. Parental karyotypes were normal, indicating a de novo chromosomal rearrangement. Following induction of labor at 39 weeks gestation a live female infant was delivered, birthweight 3310 g. The infant had generalized hypotonia, micrognathia, epicanthic folds, bilateral talipes, dysplastic ears and abnormal rolling eye movements. She was intermittently apneic. Active management was declined by the parents in view of the known chromosomal abnormality. The infant was discharged home and died on Day 24 of life. Trisomy of the short arm of chromosome 5 is very uncommon and to our knowledge this is the first reported case of an antenatal diagnosis of this condition. Various phenotypes have been described and depend on the region of the chromosome involved in the duplication. The critical region appears to be region 13. Infants with duplication of this region display a characteristic phenotype with generalized hypotonia, facial dysmorphism and developmental delay. Prognosis for these individuals is very poor. The antenatal diagnosis of trisomy 5p involving duplication of region 13 in this case enabled informed decision-making regarding the intrapartum and neonatal management of this infant.
The present study evaluated the effect of supplementing the medium used to mature equine oocytes in vitro with oestrous mare serum (EMS) or horse follicular fluid (HFF). To this end, 144 ovaries were obtained from mares aged 16-21 months and transported to the laboratory in Dulbecco's phosphate buffered saline (D-PBS) at 30 °C. Oocytes were harvested from the ovaries by slicing, and then selected for in vitro maturation (IVM) according to the number of cumulus cell layers and the characteristics of the cytoplasm. The selected oocytes were washed three times in TCM199 medium plus HEPES (TCM-199H) or in the same medium plus glutamine (TCM-199G), then matured in vitro in six study groups established according to the in vitro maturation (IVM) treatment to see possible interactions between HEPES and glutamine on other supplements: Ten percent EMS was added to two of these media and 10% HFF was added to the media in two other groups . IVM was performed at 38.5 °C for 40 h in a controlled atmosphere (5% CO 2 , 95% relative humidity). The findings indicate that the presence of EMS or HFF in the TCM-199H medium gives rise to the best results in terms of the proportions of oocytes reaching maturity (37.7% and 36.8%, respectively). The values obtained with EMS and HFF were statistically similar to each other but differed from the other treatments. The media containing glutamine led to the highest proportions of degenerated oocytes.
All coordinated: Coordination polymers are an intriguing class of materials that combines classical and modern metal‐coordination chemistry with the often complex formation of large supramolecular structures (see picture). A recent example is the formation of coordination nanotubes, in which the nature of the azine ligand profoundly defines the structure of the resulting assemblies.
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