The experiment was conducted at Lath house / Department of Horticulture and Landscape at the College of Agriculture / Tikrit University for the period from 17/3/2020 until 2/9/2020 on Catharanthus Roseus (L.) G. Don plant variety Pacifica X P Mix. The experiment was carried out using two factors under with Randomized Complete Block Design (R.C.B.D) in split plot, arranged into two factors which were put into the main plots involved applying water tension to the plant in three levels of field capacity (100%, 75% and 50%). The second factor which was placed in the sub plots, was sprayed on the shoots until completed wetness with four concentrations (0, 100, 200 and 300)mg.L−1. The results showed the superiority of (75%) field capacity and significant difference was recorded on most of the studied traits, where the leaf content characteristic of Vinblastine was recorded (114.750) mcg.−1. The root content characteristic of Vinblastine alkaloid was recorded (27.56) mcg.−1, and the root content characteristic of Vincristine (22.88) ) mcg.1 1was recorded, compared with the two water stress treatments (100% and 50%) Field capacity. Whereas, level of (100%) field capacity, outperformed the leaf content of Vincristine, which recorded (86.15) mcg.−1, which did not significantly -differentiate with the water tension treatment A2 at a level (75%) field capacity, which recorded (84.69) mcg.−1. As for the treatment of spraying with (Proline), the results showed that the treatment of B4 was superior to a concentration of 300 mg.L−1 in all studied traits (Vinblastine and Vincristine leaf and root content) compared with all concentrations.

A field experiment was conducted in the lath house of the Agricultural Research and Experiment Station of the College of Agriculture in the governorate of Kirkuk-Iraq for the period from 1/3/2022 to 1/9/2002, to evaluate the efficiency of N.Oleander L.Album Plenum in the phytoremediation of lead (Pb) contaminated soil by adding Humic acid with three levels (0,5,10) ml..pot−1 of soil directly, the design of the experiment used is R.C.B.D. The results of the mechanisms and indicators of the efficiency of the nerium oleander in the extraction of heavy metals showed that the oleander plant with the assistance of humic acid has a high ability to absorb and accumulate the lead element very effectively, and the concentration of 10 ml.pot−1 of humic increasing the concentration of the Pb in the shoot and root system (30.71, 16.83 PPM) respectively, however the concentration of lead in the control plants was (25.32 and 14.36 PPM) respectively. Translocation factor TF>1 for all humic concentrations (1.82, 1.88, 1.77), respectively, and this indicates the high ability of the oleander to absorb and accumulate heavy elements. The concentration of 10 ml.pot−1 of humic acid gave the highest value of the bioaccumulation factor (BAF) 0.85, however it was 0.64 in the control plant. The concentration of 10 ml.pot−1 of humic was also superior in giving the highest value of the concentration index (CI), which ranged 1.27, and 1.06 in the control plant.