SummaryA method is described in which a liquid suspension of sheep faeces is used as an inoculum in the in vitro determination of digestibility of feedingstuffs for ruminants. The modified method was applied to 21 samples of grass, ten of lucerne, and a variety of other food materials. The results correlated closely (r = 0·98) with the in vivo digestibilities, and the relationship between in vitro and in vivo digestibilities was represented by the equation: in vivo digestibility = in vitro digestibility × 1·003.

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SUMMARYThree in vitro methods, one enzymic and two microbial, were applied satisfactorily to the determination of the dry matter digestibility of forages, but failed when applied to a variety of concentrate feeds. The microbial methods had the advantage that the proportion of weight lost from forages during the in vitro process approximated closely with their determined in vivo digestibilities.The microbial method based on sheep faeces was as effective as that using rumen liquor in digesting ruminant feedstuff's in vitro. Since sheep faeces are readily obtained from intact animals, the faeces liquor method would seem to have a distinct advantage in use.