Wadakaha (Acorus calamus L.) is a medicinal plant of great commercial value and a potential export crop. The practice of propagating this plant based on rhizomes is inadequate to provide the planting material required for large-scale cultivation. This paper describes a tissue culture based method for mass production of Wadakaha propagules. Apical shoot meristems were cultured on MS (Murashige & Skoog, 1962) medium supplemented with BAP or kinetin(0.1-2.0 mgl-') along with IAA, IBAandNAA(0.01-1.0 mgl-l)forculture initiation. Well developed shoots were transferred to solid or liquid MS medium with BAP or kinetin (0.5-5.0 mgl-l)forshootmultiplication. Forculture initiation the medium containing BAP (0.5 mgl-l) and NAA(0.2 mgl-') was the best. Liquid media containing BAP 1.0 mgl-I and 2.0 mgl-I both gave the highest number of shoots (26 shoots/explant). The results show that BAP produced significantly more shoots than kinetin. Liquid media were more promising than solid media.
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