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An annotated list of 31 spider species of 12 families collected in Northeasten, Easten and Southern Ukraine is presented. Four speceis, Marinarozelotes manychensis (Ponomarev & Tsvetkov, 2006), Turkоzelotes kazachstanicus (Ponomarev et Tsvetkov, 2006), Zelotes atrocaeruleus (Simon, 1878), and Z. puritanus Chamberlin, 1922, are recorded for the first time from Ukraine. For Micaria coarctata (Lucas, 1846), this is the first record from the East European Plain. Nine species were recorded for the first time from Left-Bank Ukraine. To date, 1081 spider species are known from Ukraine and 757 species from Left-Bank Ukraine. Bsed on recent data, the northern boundaries of the geographic ranges of Drassyllus crimeaensis Kovblyuk, 2003 and Bassanioides caperatus (Simon, 1875) run along the northern coast of the Sea of Azov. For Marinarozelotes manychensis and Turkоzelotes kazachstanicus the northwestern coast of the Sea of Azov is the westernmost known limits of their distiribution. The range of Heriaeus horridus Tyschchenko, 1965 is delimited in the west by the right bank of the Dnieper estuary. Thirteen species are illlustrted.