No abstract

A new method for exsanguinating mice is described. The procedure is carried out under CO2-anaesthesia and involves venepuncture of the caudal (inferior) vena cava and gentle aspiration of blood into a syringe. It consistently gives large volumes of blood (1.60-2.50 ml/mouse), free from haemolysis and contamination. The technique is easy, quick and reliable.

This study tested the hypothesis that immediate washing "removes" sperm surface antibodies. Nine ejaculates with antisperm antibodies were studied. Separate portions of each ejaculate were washed by centrifugation (Shulman's method) as soon as possible after production (0-h) and 2 h later (2-h). In four samples, portions were also washed by a simple swim-up migration started at 0-h. Washed sperm were tested for surface antibodies using the immunobead binding test. The number of sperm with bound IgG antibodies at 0-h was significantly less than that at 2-h (P less than 0.001) but no significant difference was found in respect of IgA antibodies. Swim-up migration yielded results similar to those of centrifugation and had the advantage of selecting highly motile sperm. The potential application and implication of these findings are discussed.

Summary. This study investigated the clinical features which could serve as markers to identify subfertile men with antisperm antibodies including age, duration of infertility, history of abdominal or perineal surgery and previous genital trauma. Of the 162 men studied, 43 had a positive test for antisperm antibodies. A significantly greater number of these patients had a history of genital trauma and appendicectomy. Whereas the aetiological role of genital trauma in spcrmatozoal autoimmunity is readily explicable, that of appendicectomy is not. It can only be postulated that inflammation of the appendix may sometimes affect the vas deferens or that there is some inadvertent injury to the vas deferens during appendicectomy.

This study examined the effect of antisperm antibodies on sperm motility. Antisperm antibodies present in seminal plasmas with different sperm agglutinating titres were transferred passively to normal donor sperm, and the effects on movement characteristics (velocity of forward progression, amplitude of lateral head displacement, percentage progressive motility and percentage non-progressive motility) were analysed using timed exposure photomicrography. There was no significant association between sperm movement characteristics and the presence of titre of antisperm antibodies in seminal plasma. Furthermore, no differences were detected between those samples that possessed sperm agglutinating versus sperm immobilizing activity. These findings do not support the common belief that antisperm antibodies are a cause of poor sperm motility in semen.