The question of why plants release isoprene when heat stressed has been continuously debated for more than half a century. In this work we use molecular dynamics simulation techniques to directly investigate the interaction between isoprene and a model phospholipid membrane in atomic detail. It is found that isoprene partitions preferentially in the center of the membrane and in a dose dependent manner enhances the order within the membrane without significantly changing the dynamical properties of the system. At a concentration of 20 mol% isoprene (16 isoprene molecules per 64 lipid molecules) the effect of the addition of isoprene on the membrane order is equivalent to a reduction in temperature of 10 K, rising to a reduction of 30 K at 43 mol% isoprene. The significance of the work is that it provides for the first time direct evidence that isoprene stabilizes lipid membranes and reduces the likelihood of a phospholipid membrane undergoing a heat induced phase transition. Furthermore it provides a clear mechanistic picture as to why plants specifically utilize isoprene for this purpose.
Since about 1990, when sustainability became a key concept for a wide range of scientific disciplines, the need for multidisciplinary collaboration has increased. We present five illustrative cases from the long-standing environmental research work at the University of Groningen. The projects described are about hazardous materials risk, odor annoyance, energy scenario evaluation, climate decision analysis, and household consumption, respectively. The various case discussions emphasize experiences in research conceptualization, project design and execution, main findings, policy advice and surplus value, and difficulties met. Conclusions and recommendations are presented about the practice of multidisciplinary research. Finally, some challenges for research and development about environmental sustainability are discussed.
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