Thioamido, quinilineo, benzamido and benzoido group containing heterocycles created their own identity and importance in pharmaceutical, medicinal, agricultural and drug sciences. Thioamido and benzamido heterocyclic compounds showed noticeable and remarkable applications in industrial, pharmaceutical, medicinal and drug chemistry. Hence taking all these facts into consideration it was thought interesting to synthesize N-(7-substitutedthiocarbamidoquinoline-4-yl )-N, N-diethyl-pentane-1,4-diamines by interacting N-(7-chloroquinoline-4-yl)-N,N-diethyl-pentane-1,4-diamine with variousthiourea in isopropanol medium .The justification and identification of the structure of these newly synthesized compounds had been established on the basis of chemical characterization, elemental analysis and through spectral data The antimicrobial activities of this compounds were screened against E. coli, S. typhi, S. abony, P. aeruginosa, B. subtilis, A. niger, and C. albicons pathogens which are hither to unknown.