Commercial kiwifruit production often requires substantial inputs for successful pollination. Determining the length of time that female flowers can be successfully pollinated can aid management decisions concerning pollination enhancement. The purpose of this research was to determine the effective pollination period (EPP) for 'AU Golden Sunshine' and 'AU Fitzgerald '. Either 30 (2013'. Either 30 ( ) or 32 (2014'. Either 30 ( , 2015 flowers of 'AU Golden Sunshine' were hand pollinated each day for 1 to 5 (2013) days after anthesis (DAA) or 1 to 7 DAA (2014, 2015), and then isolated to prevent open pollination. Anthesis was considered the day the flower opened. Similarly, 'AU Fitzgerald' flowers were pollinated and then isolated 1 to 6 DAA in 2013 and 1 to 7 DAA in 2015. For 'AU Golden Sunshine' in 2013, fruit set was consistent over the 5-day period, but fruit weight, fruit size index, and seed number decreased between 1 and 3 and 4 and 5 DAA. In 2014, fruit set decreased between 1 and 6 and 7 DAA, whereas fruit weight, fruit size index, and seed number each decreased in a linear trend. In 2015, fruit set also decreased between 1 and 6 and 7 DAA, whereas all other responses decreased linearly. Based on fruit set in 2014 and 2015, the EPP for 'AU Golden Sunshine' was 6 DAA. The EPP for 'AU Fitzgerald', however, was more variable. In 2013, fruit weight, fruit size index and seed number decreased between 1 and 4 and 5 and 6 DAA, suggesting that the EPP was 4 DAA. In 2015, fruit set remained consistent over the 7-day period with fruit weight, fruit size index, and seed number decreasing linearly. Differences in temperature and the alternate bearing tendency of kiwifruit species likely contributed to the discrepancies between the years for the EPP. For each cultivar, reductions in fruit weight, size, and seed number were observed before an observed decrease in fruit set. Greater fruit weight, size, and seed number were observed when flowers were pollinated within the first few DAA, with results varying thereafter.
