Results obtained from random amplified polymorphic DNA (RAPD) analysis of four species of the genus Eucalyptus revealed that the number of fragments amplified for each of the five studied primer varied between 13 and 14 fragments and their size ranged from 95 to 1055 bp. The total number of bands was 67, total number of monomorphic bands was 32, total number of polymorphic bands was 35, and the polymorphism percentage was 52.2%. The results indicate that 16 positive and 13 negative markers were detected. The marker fragments size ranged between 175 to 630 bp for the negative markers and 235 to 945 bp for the positive markers.