The use of antibiotics in the treatment of livestock has increased the productivity of the meat and dairy industry. The flip side of the effectiveness of drugs of this group is the presence of side effects, which have caused concern about the widespread use of antibiotics and cause the need to control the residual content of antibacterial agents in dairy products. From soil samples collected in 7 regions of Kazakhstan, 14 microbial isolates were isolated and identified as B. licheniformis, B. sonorensis, B. cereus, B. simplex, B. thuringiensis, B. pumilis, B. mojavensis, B. subtilis, B. atrophaeus, B. paralicheniformis. Sensitivity testing of the isolated strains showed that the strains were susceptible to 13 antibiotics belonging to lincosamide, ansamycin, quinolone, macrolide, fluoroquinolone, tetracycline, nitrobenzene, aminoglycoside, glycopeptide and beta-lactam antibiotics. The proteolytic strain Bacillus licheniformis T7 seems promising as a test culture. It is highly sensitive to antibiotics, grows rapidly on a variety of nutrient media, is alkaline and spore-forming and can be cultured at 37-55°C. The peculiarity of the strain to change the pH of the medium from 5.0 to 7.0 and above makes it possible to use bromcresol purple as a growth-detecting dye. This has been shown in experiments using antibiotic milk samples on LB agar and LB broth pH 5.0. In the absence of growth-inhibiting antibiotics, the culture of B. licheniformis T7 grows vigorously, which causes a pH shift to 7.99 and causes the color of bromocresol purple to change from yellow to purple. The results showed promise for the strain Bacillus licheniformis T7 to be used as a bacterial culture in the development of a microbiological test for the detection of antibiotics in milk.

Antimicrobial agents are used in animal husbandry for the prevention and treatment of diseases in farm animals and have become an indispensable aspect of commercial livestock production. Antimicrobial therapy is well-established as a component of comprehensive preventive measures aimed at minimizing diseases in farm animals and has been incorporated into procedures directed at promoting livestock growth and productivity. Bacillus licheniformis strain T7 is susceptible to the antibiotics clidamycin, rifampicin, erythromycin, ciprofloxacin, tobramycin, tetracycline, penicillin, streptomycin, and chloramphenicol, has good sporogenic properties, grows rapidly on nutrient agar at 30-55 °C, pH 5.5–8.0, and can serve as a test culture in a microbial inhibitor test. Bacillus licheniformis T7 spores were obtained by growing the culture in Difco sporulation medium and then inactivating the vegetative cells at 90 °C for 20 minutes. Endospores of Bacillus licheniformis T7 co-polymerized with nutrient agar and germinated at 55 °C for 3–3.5 h, resulting in a pH shift from 5.5 to >6.5, which can be measured with an acid-base indicator. Bromocresol purple was suggested as a pH indicator for use in a microbiological inhibition test, wherein the presence of antibiotics or other compounds impeding the development of a microbiological culture could be determined by a change in the agar's color. A microtube- and plate-based microbiological inhibitor test prototype has been developed.

The ability to identify vegetation types is very important for the study of flora biodiversity. DNA barcoding makes it possible to standardize the identification procedure by reference DNA regions. The most popular sequences for molecular identification and phylogenetic studies are Internal Transcribed Spacer (ITS) sequences. ITS are widely used as a phylogenetic marker to classify plants at various taxonomic levels, including genus, species, and subspecies.            29 samples of rare and endangered plant species were collected from Aksu-Zhabagly State Nature Reserve, Sayram-Ugam National Park and Berikkara Tract State Nature Reserve. DNA was isolated by the CTAB method, oligonucleotides flanking the ITS region of the nuclear DNA region were selected and synthesized. Sequencing was carried out and ITS sequences of the region were obtained for 29 species of rare and endangered plant species in Kazakhstan.