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The current study was conducted to estimate the effect of soil and growth climatic conditions on the vitamin content of red clover (Trifolium pratense L.). Further, in vitamins, the content of ascorbic acid and vitamin A (provitamin – carotene) was estimated from the aerial parts of the clover. Ascorbic acid is a powerful antioxidant, antiviral, and antitumor vitamin while vitamin A is a useful vitamin for eyesight. Ascorbic acid content in the red clover tissues was determining potassium hexacyanoferrate method while Provitamin A and total carotenoid was estimated by spectrophotometric method. Results of the study revealed that the highest content of vitamins C was found in the plant grown under the climatic conditions of the southern taiga subzone of the Atninsky and in the zone of deciduous forests (Apastovsky and Kamsko-Ustinsky districts) while the plant is grown under the coniferous-deciduous forests (Zelenodolsky district) climatic conditions are a rich source of vitamin A. From the results of the study, it can conclude the soil and growing conditions especially temperature regime and soil moisture affected the level of vitamins in red clover.