Studies of ~he role and nature of the cholinergic receptors acting on the secretion of isolated perfused rat pancreas have shown the following: The infusion of acetylcholine at a dose of 2.5 ~M in the presence of a concentration of glucose of 1.5 g/l, provoked a first phase of immediate and important stimulation of the secretion of insulin; this initial peak of insulin secretion was followed by a second phase during which a new less intense stimulation occurred; the latter was followed by an inhibition appearing at a time that depended on the pancreas used. At a dose of 0.5 ~M of acetylcholine, the first phase of stimulation always appeared; during the second phase some par/creases were inhibited, others remained stimulated. --The peak of insulin secretion obtained by stimulation with acetylcholine during the first phase was dose related. --Eserine intensified the effects of acetylcholine. --The presence of glucose was essential for the insulinsecretory action of acetyleholine. The muscarinic nature of the cholinergie receptors implicated in the secretion of insulin was demonstrated by the use of: --Atropine which completely blocked the effects of acetylcholhm, --Musearine which produced the same effects as aeetylcholine on our pancreas preparation, effects which were equally inhibited by atropine. The cholinergie receptors of the endocrine beta cell of the islet of Langerhans of the pancreas are therefore of the muscarinic type.

No abstract

Insulino-s6cr6tion 6tudi6e sur le pancr6as isol6 et perfus6 du rat II. Action des cat6cholamines et des substances bloquant les r6cepteurs adr6nergiques

No abstract

No abstract