This experimental work aims to evaluate the efficacy of supplying prostaglandin F2α (PGF2α) and gonadotropin-releasing hormone (GnRH), propylene glycol (PG), or their combination on reproductive performance and some blood parameters of postpartum (PP) Egyptian buffaloes. A total of 32 postpartum Egyptian buffaloes were divided into 4 groups. Buffaloes in group 1 (G1) had no treatment. Group 2 (G2) was injected with PGF2α (at Day 0, calving day) and GnRH (At Day 15 PP). Animals in group 3 (G3) received oral doses of PG (300 ml) at 0-, 3-, 6-, 9-, 12-and 15-days PP. Animals in group 4 (G4) were given combination of G2 and G3 treatments. The results showed a significant improve in all reproductive traits of buffaloes treated with hormones, PG and their combination compared to the control group, exemplified with significant increase in blood total protein, albumin, globulin, total lipid, and cholesterol, and insignificant increase in triglyceride. The levels of creatinine and transaminases were decreased compared to control group. In conclusion, PGF2α + GnRH treatment, PG treatment, or their combination improved the reproductive performance and blood parameters of the postpartum Egyptian buffalo.