Molecular tools were used to analyze the genetic diversity and population structure of Xanthomonas oryzae pv. oryzicola, the bacterial leaf streak pathogen of rice in the Philippines. Representative pathogen strains were selected and used to assess resistance in rice germplasm. A partial genomic library of X. oryzae pv. oryzicola was constructed, and a 459-bp clone containing the repetitive DNA element R41 was selected as a probe for restriction fragment length polymorphism (RFLP) analysis and sequenced. R41 shared 44% sequence homology with the putative transposase gene of IS1112, an insertion element cloned from X. oryzae pv. oryzae. Using R41 as a probe for RFLP analysis, 26 band profiles were discerned in a collection of 123 strains of X. oryzae pv. oryzicola. Analysis of PstI digestion patterns of DNA from the same collection resolved 36 haplotypes. Several clusters of strains were detected after grouping of data based on either pR41 as a probe or Pst1 digestion patterns. However, based on bootstrap analysis, the clusters were not robust. Genetic diversity was high for the entire collection as well as within spatially and temporally defined subsets of strains. Even a set of strains collected from a single site at a single time was highly diverse. Strains representing the different DNA types were inoculated to a set of diverserice cultivars. Consistent rice varietal groupings were obtained from disease reaction data, but there was no correlation between pathogen isolate cluster and host reaction across inoculation trials. Isozyme group I of rice, representing tropical japonica and javanica germplasm, is a promising source of resistance to bacterial leaf streak.

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In anticipation of the Philippines being a major producer of organic bananas, this study was conducted to provide a quantitative basis for certain practices in organic farming. The nutrient supplying capacity of banana residues in combination with leguminous materials and chicken manure was investigated in composting studies. Changes in the chemical composition of ten formulations of banana residue-based compost involving leguminous plants (Sesbania rostrata, Flemingia macrophylla, Arachis hypogea) and chicken manure were analyzed periodically during a composting period of 16 weeks. Results showed that combinations of banana residues (BnR) and chicken manure or leguminous plants were highly decomposed compared to untreated BnR. The use of leguminous plants and/or chicken manure enhanced the composting process significantly compared to the effect of Bioquick. The compost piles were characterized by increases in pH, total N and total P, and decreases in total K, total carbon and C/N ratio with time. Notably, BnR+chicken manure attained a C/N ratio of 15 at 4 weeks, while the BnR+leguminous materials reached such a low C/N ratio at 8–16 weeks. An incubation study was conducted under greenhouse conditions for 24 weeks. It was designed to follow the dynamics of nitrogen (N), phosphorus (P) and potassium (K) availability in two clay soils (Antipolo and Lipa) amended with five compost formulations (BnR alone, BnR+Sesbania prunings, BnR+Flemingia prunings, BnR+peanut stover and BnR+chicken manure) and with uncomposted banana residue at an application rate of 20 Mg ha−1. Results showed that net N mineralization occurred in soils amended with BnR+chicken manure and BnR+leguminous materials, which had C/N ratios ranging from 12 to 16. Net N immobilization during the earlier period of incubation was observed in uncomposted and composted banana residues with a C/N ratio of 68 and 24, respectively. Significantly higher net P mineralization was obtained only in soils amended with BnR+chicken manure. An abrupt increase in exchangeable K was observed in all treatments 2 weeks after the incorporation of organic residues. Higher available K in pure BnR treatments (uncomposted or composted) exhibits the inherently high K content of banana residues.

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