Present paper deals with study of impact of physical factors on extracellular lipase activity of some dominant oilseed-borne fungi. In present investigation, seed-borne fungi were isolated from abnormal oilseeds by agar plate method. Of the 11 genera and 17 species of fungi isolated, 10 dominant fungi were selected to study their lipase enzyme activity. Various parameters such as temperature, pH, light and incubation period were studied in order to determine the optimum conditions for lipase production of ten dominant fungi. The lipase present in the broth was assayed by cup-plate method. It is revealed from the result that lipase activity of storage fungi was found to be optimum at temperature 20⁰C, pH 6.5, incubation period of 20 days and continuous light.

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The present paper deals with the study of the impact of environmental factors like temperature and relative humidity (R.H.) on severity of disease, spore germination, cellulase and pectinase enzyme activity of Colletotrichum gloesporioides. Development of anthracnose (Colletotrichum gloesporioides rot) was found to be very less at low temperature (100 C) and low R.H. (30%) whereas; it was highest at 25 0C and at 100% R.H.

This work represents the study of mass attenuation coefficient, total atomic cross section, total electronic cross section, effective atomic number, effective electron density and half value layer for selected three polymers : Polyacetylene (C2H2n), Polyethelyne (C2H4n), Polypyrrole (C4H5n) seven Saturated fatty Acids : Arachidiec Acids (C20H32O2), Capric Acid (C10H20O2), Caprylic Acid (C8H16O2), Cerotic Acid (C26H52O2), Lauric Acid (C12H24O2), Myristic Acid(C14H28O2), Plamatic Acid(C16H32O2) and Six Unsaturated fatty Acids : Arachidonic Acid (C20H32O2), Decosahexaaenic Acid (C22H32O2), Eliadic Acid (C18H34O2), Lenoleic Acid (C18H32O2), Erucic Acid (C22H42O2), Oleic Acid(C18H34O2) using NIST-XCOM and Geant4 Monte-Carlo simulation at the energy range 122 keV to 1330keV. Successfully designed narrow beam geometry in Geant4 code for low energy electromagnetic interaction study. We found the errors within 0.001% in the determined results of mass attenuation coefficient and showed Geant4 isa powerful toolkit for measurement of radiation interaction parameter with matter. The variation in effective atomic number and effective electron density with incident photon energy were observed due to the prevalence of photon interaction processes. The Monte-Carlo simulations and theoretical simulation results have been showed that the polymers have good shielding properties as compared with saturated and unsaturated fatty acids. From obtained results, it was noticed that Geant4 Monte-Carlois in good accord XCOM database.

About 90% of the world food crops are being produced by using seeds. Healthy seeds are important for production of healthy crop and these seeds are also responsible for disease transmission. It happens either in field or in post-harvest storage condition. Due to these seed borne fungi, seed get deteriorated which may cause a great economic loss as well as several types of abnormalities occur in seeds. Major storage grain fungi contaminated with mycotoxin, mycotoxins contaminants of fungal origin occurring worldwide and characterized by its acute and chronic toxic effects on human health. In the present attempt were made to study the antifungal activity of leaf extract against mycotoxin producing fungi. Fungi isolated from stored seed grains like  cereals, pulses, infected oil seeds were collected from from Marathwada region of Maharashtra, India. Antifungal activity of leaf extract of Calotropis procera, Azadirachta indica, Ocimum sanctum, Withania somnifera and Datura metel were tested against Alternaria alternata, Aspergillus flavus, Aspergillus ochraceus, Fusarium gramineorum and Penicillium citrinum by using 96-well plate method. Leaf extract showed remarkable activity against tested fungi at different concentrations as Minimum Inhibitory Concentration. The leaf extract of Calotropis procera and Azadirachta indica, have good potential antifungal properties against different mycotoxin producing fungi.