According to the latest statistics, grapes are one of the largest fruit crops worldwide. In this regard, it is important to consider all factors influencing quality of grapes and wine. In the last years, scientist focused on the study of mycotoxins that can influence the quality of wine. It is considered that toxins produced by moulds, causing significant economic losses, affect approximately one quarter of the world grape production. If the selective sorting of infected grapes is not done adequately, wine will present a major risk to consumers, mycotoxins being considered by the "International Agency for Cancer Research" a carcinogenic compound. The main mycotoxins monitored in this study come from Aspergillus sp., and are represented by aflatoxins B1, B2, G1, G2 and ochratoxin A. This study purpose is to develop a faster method for the analysis of mycotoxins, in order to increase rapidity and efficiency for the evaluation of the degree of infestation in wine. The purposed method is using an on-line large volume injection coupled to pre-concentration of sample (SPE) which is directly transfer to the ultra-high-pressure liquid chromatography (UHPLC) column for separation and the detection by means of the fluorescence detector (FLD). As the maximum tolerated level for mycotoxins in wines is 2 ppm, this method is able to detect under this limits of quantification with RSD below 2%.

Romania has a long tradition regarding the production of wine rustically or household products. Each householder has the possibility for making its own wine from its own grape vines. Most of them are made and kept using almost no interventions or treatments no corrections or added substances, which could protect them from eventual diseases, which may appear during the winemaking process. The grapes are collected manually by each family alone and it can be called is itself a ritual specific to the each area and a symbol of autumn. In this context, several samples of wine were collected from households located in five Romania eastern counties (Iaşi, Vaslui, Galaţi, Brăila, Ialomiţa and Tulcea). The samples were neither filtered, nor treated against the developing secondary fermentations. This fact increases the risk of infestation with mycotoxins. Twenty samples belonging to the aforementioned regions were analysed using the direct real time method (DART). This method is very fast and does not require any prior preparation of the samples. The goal was to identify the aflatoxins B1, B2, G1 and G2 that are known to be frequently present in wines. From the total of the analysed samples, in four of them it was detected a concentration greater than 20 μg/kg.