The metabolism of a new fluorinated fungicide [N-ethyl-N-methyl-4-(trifluoromethyl)-2-(3,4-dimethoxyphenyl)benzamide] by Acer pseudoplatanus cells was studied concurrently through the use of 19F NMR and with 14C-labeled compound. Approximately 10 fluorinated compounds were observed by 19F NMR in the culture; 5 of them were identified. The detection limit was <1 μM. Methanol appeared to be a useful solvent as it was able to dissolve the lipophilic active ingredient as well as the more polar derivatives. However, the use of chloroform allowed a better separation of the NMR signals for the derivatives fairly soluble in this solvent. The metabolism of the studied compound mostly occurred through ring demethoxylation and N-dealkylation. The more lipophilic derivatives diffused into the culture medium; in contrast, the more hydrophilic ones remained inside the cells. This work shows that the sensitivity and high resolution power of liquid 19F NMR spectroscopy confirm its potential usefulness to study the metabolism of xenobiotics. Keywords: Acer pseudoplatanus L.; 19F NMR; fungicide; metabolization; plant cells