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ESSAI DE TAXONOMIE DES AMIBES LIBRESTaxonomy-trial of the free-living amoebae belonging to the genus Acanthamoeba (Protozoa, Amoebida) by means of the cyst-features in scanning electron microscopy.SUMMARY. In order to verify the contribution of scanning electron microscopy to the taxonomic study of free-living amoebae, the authors studied at the encysted stage 21 strains of free-living amoebae representing 20 species (1 Naegleria gruberi, 1 Tetramitus rostratus and 18 Acantha moeba sp.). The method utilized is satisfactory for distinguishing the different genera. Using optical methods, it is possible to differentiate three groups (I, II, III) of Acanthamoeba, including 3, 10 and 5 species, respectively. The results obtained with scanning electron microscopy, how ever, are less precise. In particular, group II can be divided into only four sub-groups and group III into only two sub-groups and one species, A. culbertsoni. The method remains valid for group I.

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RÉSUMÉ. Nous avons soumis des oeufs d'Aedes aegypti à l'action de différentes souches de spiroplasmes isolées de moustiques, tant en Savoie française qu'à Taïwan. Seule la souche de spiroplasmes SP7, originaire d'Armigeres subalbatus de Taïwan, témoigne d'un réel effet pathogène sur l'évolution des larves issues des oeufs d'Ae. aegypti, sans altération apparente du sex-ratio, ni transmission du pouvoir infectieux aux imagos issues des lots exposés. Les auteurs, après avoir présenté les différents résultats obtenus, évoquent les difficultés, notamment culturales liées à l'emploi des spiroplasmes. SUMMARY. The authors did study the experimental effects on Aedes aegypti ova of different Spiroplasma strains, isolated from mosquitoes in french Savoie and in Taïwan. The SP7 strain, from Armigeres subalbatus (Taïwan), demonstrates a true pathogenic effect on the larval evolution, without sex-ratio modifications, nor bacterial transmission to the adult mosquitoes. The authors present their results and emphasize the difficult use of Spiroplasmas sp.

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