Autism Spectrum Disorders (ASD) comprise a group of heterogeneous and complex neurodevelopmental disorders. Genetic and environmental factors contribute to ASD etiology. DNA methylation is particularly relevant for ASD due to its mediating role in the complex interaction between genotype and environment and has been implicated in ASD pathophysiology. The lack of diversity in DNA methylation studies in ASD individuals is remarkable. Since genetic and environmental factors are likely to vary across populations, the study of underrepresented populations is necessary to understand the molecular alterations involved in ASD and the risk factors underlying these changes. This study explored genome-wide differences in DNA methylation patterns in buccal epithelium cells between Mexican ASD patients (n = 27) and age-matched typically developing (TD: n = 15) children. DNA methylation profiles were evaluated with the Illumina 450k array. We evaluated the interaction between sex and ASD and found a differentially methylated region (DMR) over the 5′UTR region of ZFP57 and one of its targets, RASGRF2. These results match previous findings in brain tissue, which may indicate that ZFP57 could be used as a proxy for DNA methylation in different tissues. This is the first study performed in a Mexican, and subsequently, Latin American, population that evaluates DNA methylation in ASD patients.

An excessive water cut or high gas/oil ratio in a production interval presents a major concern in sustaining oil production, often requiring fast and efficient workover solutions to enhance the oil recovery process. Wells in the Cantarell field, a mature depleted field in the Bay of Campeche in the Gulf of Mexico, are facing drastic decreases in their production and, depending on producing zone, an increase in either water cut or gas/oil ratio. Other developed fields in Mexico's Region Marina, such as the Ku-Maloob-Zaap, have constantly increased their hydrocarbon production through the years with an incipient increase in water and gas increments.The high water cut and gas increments have had a strong impact on the production strategy, opening the opportunity for application of non-conventional, innovative, and engineered solutions to isolate or abandon production intervals invaded by gas or water and continue production from upper or deeper zones. The pay zones consist of naturally fractured, vugular carbonates with permeability as high as 5 Darcies, from Paleocene, Cretaceous, and Jurassic formations. Their characteristics present the following challenges that need to be overcome to succesfully achieve the required isolation: Loss of fluid circulation, which is necessary for well control and also negatively affects the fluid placement in the workover jobs.  High gas presence, gas cap driven, which is a major concern on surface.  Lack of primary zonal isolation, resulting in migration of water or gas to the productive intervals. This paper summarizes the non-conventional technologies and techniques applied to isolate the water and gas producing intervals and their synergistic performance: reticulated gel, lost circulation fiber tecnologies and gastight slurries integrated in an engineered solution. Results from field cases demonstrate the design, execution, and evaluation of these applications.

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Introduction. The study of autistic spectrum disorders (ASD) at the genetic level is extremely important to understand their origin. In Mexico, there are few works addressed from this perspective. Objective. We investigated the role of the Brain Derived Neurotrophic Factor ( BDNF ) gene variant rs6265 G/A for single nucleotide polymorphism analysis in Mexican children with ASD using a case-control association design. Method. We made a pilot study by case-control analysis adjusting by gender, age, and ancestry. Results. Our study found no association between the BDNF rs6265 gene polymorphism and ASD [ p = .419, OR = 1.597 (.514, 4.967)]. Discussion and conclusion. Worldwide, the results of case-control association studies with the rs6265 of BDNF are controversial and do not always replicate. This may be due to the ethnicity of our population and additional factors not studied in the present work. Our study suggests that the SNP rs6265 is not contributing for ASD susceptibility in Mexican population.