Although HIV-1 subtype A has predominated in Russia since the end of the 20th century, other viral variants also circulate in this country. The dramatic outbreak of HIV-1 subtype G in 1988-1990 represents the origin of this variant spreading in Russia. However, full genome sequencing of the nosocomial viral variant and an analysis of the current circulating variants have not been conducted. We performed near full-length genome sequencing and phylogenetic and recombination analyses of 11 samples; the samples were determined to be subtype G based on an analysis of the pol region. Three samples were reliably obtained from patients infected during the nosocomial outbreak. The other 8 samples were obtained from patients who were diagnosed in 2010–2015. Phylogenetic analysis confirmed that a man from the Democratic Republic of the Congo was the origin of the outbreak. We also found that currently circulating viral variants that were genotyped as subtype G according to their pol region are in fact unique recombinant forms. These recombinant forms are similar to the BG-recombinants from Western Europe, particularly Spain and Portugal. The limitations of subtyping based on the pol region suggest that these viral variants are more widespread in Europe than is currently supposed.

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Aim of research: to detect a diagnostic value of cumulative clinical assessment of systemic inflammation markers in monitoring a course of HIV infection. Materials and methods. The research is implemented in a sample of 162 HIV patients. The sample is divided into two groups: 1 — receiving HAART (n=88), 2 — not receiving HAART (n=74). The content of lipopolysaccharidebinding protein (LBP), procalcitonin and cytokines (TNF-a, IL-1b, IL-6, IL-8, IL-10, IFN-g, IFN-a) are detected in serum by solid-phase enzyme-linked immunosorbent assay. The number of CD4+-lymphocytes was determined by flow cytometric techniques. Results. Significantly high LBP concentration, in comparison with healthy persons’ indices, is found in both groups of the sample. The content IFN-g and IFN-a is significantly high in majority of HIV patients of the sample regardless of the therapy received. HIV patients with low content of CD4+ lymphocytes, have the indications of more severe systemic inflammation accompanied by enhanced production of anti-endotoxin proteins, and their cytokine profile is characterized by more expressed proinflammatory orientation than in HIV patients with high CD4+ lymphocytes indices. The LBP level may be treated as an indirect criterion of immune suppression intensity in HIV infection.

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Проведена оценка состава микрофлоры слизистой ротоглотки и биохимических маркеров эндотоксемии с определением содержания LPS-Р в крови у 100 BИЧ-позитивных пациентов, получавших антиретровирусную терапию. Выявлена интенсивная колонизация слизистой ротоглотки условно-патогенной микробной флорой в высоких количественных титрах и более чем 14-кратное превышение уровня LPS-Р. Добавление к терапии на 4 недели аминодигидрофталазиндион натрия («Галавит») обеспечило достоверное снижение концентрации LPS-Р в крови и нормализацию микробного состава слизистой ротоглотки у большинства пациентов с ВИЧ.