A comparative study of total protein and albumin in man, monkey, dog and rat employing different analytical methodsSummary: In the present study, serum albumin was determined colorimetrically in three animal species and in man with the help of four currently used dye-reagents (protein binding reagents). The results were compared with those obtained from corresponding electrophoretic and biuret determinations, using Versatol as a standard-control throughout. Using 2-(4-hydroxyazobenzene)-benzoic acid and bromocresol purple as reagent for the albumin determinations, species-specific differences from the electrophoretic results were found. No such differences occurred with bromocresol green, except in rats.There was no significant difference in albumin concentrations between man, monkeys and rats. Total protein concentration was only similar in man and monkeys.

Die vorliegende Studie behandelt die Problematik der photometrischen Proteinbestimmung in Leberhomogenaten in Anwesenheit steigender Fettmengen und zeigt die dabei auftretenden Fehlermöglichkeiten auf. Es wird vorgeschlagen, diese Fettrübungseinflüsse entweder durch Detergenzzusatz (Desoxycholat) oder durch Verwendung von Spektralphotometern mit speziellen Meßanordnungen für Trübungsmessungen zu unterdrücken. The influence of fat on the determination of protein by the biuret and the Folin-Ciocalteau methods in liver homogenatesThe present study deals with the spectrophotometric determination of protein in liver homogenates and the errors of determination liable to occur due to clouding of the test sample by the presence of lipids. Two ways of suppressing this source of error are described: the addition of a detergent (desoxycholate) to the test sample to clear the clouding, and the use of appropriate optical equipment to correct the scattering effect Of the clouding on the light beam being measured.