The leafhoppers Orosius argentatus (Evans), Austroagallia torrida (Evans) and Batracomorphus angustatus (Osborn) were used in transmission tests to determine their vector status for the phytoplasma associated with Australian lucerne yellows (ALuY). Caged, seed-grown lucerne plants were monitored for foliar symptom expression after feeding by leafhoppers transferred from ALuY symptomatic lucerne plants. Twelve of 25 plants developed phytoplasma disease-like symptoms including stunting and yellowing. The most pronounced foliar symptoms were displayed by five plants that had been fed on by O. argentatus and four plants that had been fed on by A. torrida. One plant, fed on by O. argentatus , showed the distinctive root symptoms of ALuY . A phytoplasma was identified by electron microscopy in two plants fed on by O. argentatus and one by A. torrida. For each group of plants that had been fed on by a single leafhopper species, one plant was phytoplasma positive as determined by the polymerase chain reaction (PCR) using universal primers. The phytoplasma detected by PCR in the plant fed on by A. torrida was identified by restriction fragment length polymorphism (RFLP) analysis as the tomato big bud (TBB) phytoplasma. The PCR product from two plants fed on by B. angustatus and O. argentatus were too faint for RFLP analysis. PCR assays were conducted on DNA extracted from the head and thorax of each leafhopper species from transmission tests and from field-collected insects, but no phytoplasma DNA was detected. These findings suggest O. argentatus is a vector of the ALuY pathogen and A. torrida is a vector of the TBB phytoplasma.

The effects of 5 foliar-applied fungicides on seed yield of faba bean (Vicia faba) cv. Fiord were studied over 3 years at Tamworth in northern New South Wales. In 2 seasons when the diseases chocolate spot (Botrytis fabae) and rust (Uromyces viciae-fabae) were significant, 5 applications of foliar fungicides after flowering increased yield, by up to 1.6 t/ha in 1990 and nearly 0.9 t/ha in 1992, compared with the unsprayed treatment.Mancozeb, dichlofluanid, and tebuconazole were the most effective fungicides for preventing yield reduction, and vinclozolin and procymidone had little or no effect. Mancozeb and tebuconazole were effective in reducing the severity of both diseases, whereas procymidone was only active against chocolate spot. Differences between the most effective fungicides when applied 5 times or twice (at early and mid flowering) were seldom significant. Seed yields following 2 applications of tebuconazole were significantly higher than from 1 application, but for mancozeb, 2 applications were better than 1 in 1992 only. It was estimated that rust accounted for most of the yield loss in 1990 and 1992, and did so mainly by reducing seed size. Application of mancozeb early and during late flowering provided an effective and economical increase in grain yield in 1990 and 1992.

Foliar and root symptoms are described for Australian lucerne yellows (ALuY), a disease common in Australian lucerne seed crops. A phytoplasma was detected in plants exhibiting symptoms, but not in symptomless lucerne plants. Oligonucleotide primers specific to the phytoplasma 16S-23S rRNA intergenic spacer region (SR) were used in polymerase chain reaction (PCR) assays on DNA extracted from lucerne plants with and without symptoms. Identical restriction fragment length polymorphism (RFLP) enzyme profiles were obtained for PCR products amplified from 10 yellowsaffected lucerne samples. RFLP profiles obtained for four restriction enzymes were different from those of the tomato big bud (TBB) phytoplasma. ALuY phytoplasma PCR products were sequenced to determine phylogeny and were found to fall within the faba bean phyllody phytoplasma group, or phytoplasma group 16srII. Transmission electron microscopy revealed phytoplasmas in the phloem of yellows-affected plant samples, but not in symptomless plant samples. Fungal, bacterial and viral agents in the aetiology of Australian lucerne yellows were ruled out.

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